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Evaluation of Cell-Material Interactions on Newly Designed, Printable Polymers for Tissue Engineering Applications

Authors

  • Esther C. Novosel,

    1. Institute for Interfacial Engineering IGVT, University of Stuttgart Nobelstraße 12, 70569 Stuttgart, (Germany)
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  • Wolfdietrich Meyer,

    1. Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP Geiselbergstraße 68, 14476 Potsdam-Golm, (Germany)
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  • Nadine Klechowitz,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology Nobelstraße 12, 70569 Stuttgart, (Germany)
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  • Hartmut Krüger,

    1. Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP Geiselbergstraße 68, 14476 Potsdam-Golm, (Germany)
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  • Michael Wegener,

    1. Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP Geiselbergstraße 68, 14476 Potsdam-Golm, (Germany)
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  • Heike Walles,

    1. Institute for Tissue Engineering and Regenerative Medicine University of Würzburg Röntgenring 11, 97070 Würzburg, (Germany)
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  • Günter E. M. Tovar,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology Nobelstraße 12, 70569 Stuttgart, (Germany)
    2. Institute for Interfacial Engineering IGVT, University of Stuttgart Nobelstraße 12, 70569 Stuttgart, (Germany)
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  • Thomas Hirth,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology Nobelstraße 12, 70569 Stuttgart, (Germany)
    2. Institute for Interfacial Engineering IGVT, University of Stuttgart Nobelstraße 12, 70569 Stuttgart, (Germany)
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  • Petra J. Kluger

    Corresponding author
    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology Nobelstraße 12, 70569 Stuttgart, (Germany)
    2. Institute for Interfacial Engineering IGVT, University of Stuttgart Nobelstraße 12, 70569 Stuttgart, (Germany)
    • Fraunhofer Institute for Interfacial Engineering and Biotechnology Nobelstraße 12, 70569 Stuttgart, (Germany).
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  • We would like to thank Dr. Kirsten Borchers and Dr. Monika Bach for scientific discussion and proof reading, Dr. Michaela Müller for performing the contact angle measurements, as well as the Fraunhofer Gesellschaft and the Landesgraduiertenförderung Baden-Württemberg for funding the project.

Abstract

The generation of advanced scaffolds for tissue engineering is increasingly implemented by rapid prototyping techniques and therefore designed materials. Two novel polymeric networks based on newly synthesized carboxyfunctionalized polyacrylates were created to fit the requirements of 3D printing processes regarding viscosity and their surface tension. The polyacrylates presented carboxylic groups either without any spacer, or, in the case of mono-(2-acryloxyethyl)-succinate, with a substantial spacing from the polymer backbone. The material properties were characterized with rheometry, FTIR, XPS, and contact angle measurements. Polymer surfaces were prepared and seeded with primary human dermal microvascular endothelial cells (HDMEC). Cell–material interaction with the different polymer surfaces were investigated by light microscopy, water soluble tetrazolium-1 (WST-1) viability assay, fluorescein diacetate/propidium iodide (FDA/PI) staining, and acetylated low-density lipoprotein (acLDL) assay. Thus, the cell confluence, viability, and functionality of the HDMEC were evaluated. The non-spaced methacrylic acid derivative was proved in cell culture regarding its biocompatibility. Along with its appropriate physical properties, the novel network-generating polymeric blends constitute promising candidates for future scaffold design in tissue engineering.

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