N. Krithica and V. Natarajan equally contributed for the work.
Article first published online: 10 OCT 2011
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Engineering Materials
Volume 14, Issue 4, pages B149–B154, April 2012
How to Cite
Krithica, N., Natarajan, V., Madhan, B., Sehgal, P. K. and Mandal, A. B. (2012), Type I Collagen Immobilized Poly(caprolactone) Nanofibers: Characterization of Surface Modification and Growth of Fibroblasts. Adv. Eng. Mater., 14: B149–B154. doi: 10.1002/adem.201180035
Authors wish to acknowledge Dr. V. R. Giridev, Lecturer, Textile Technology, Anna University, Chennai for extending his support for electrospinning of PCL nanofibers. Authors thank the Council of Scientific and Industrial Research (CSIR) for financial support under Young Scientist Award (YSA) project scheme.
- Issue published online: 4 APR 2012
- Article first published online: 10 OCT 2011
- Manuscript Revised: 10 AUG 2011
- Manuscript Received: 13 MAY 2011
Poly(caprolactone) (PCL) electrospun nanofibers were modified by aminolysis and collagen was immobilized on the aminolysed PCL nanofibers. Considering low immunogenic response collagen elicits, immobilization of the same is anticipated to enhance the tissue engineering application of the PCL nanofibers. Amino groups were introduced into PCL nanofibers through aminolysis process. Aminolysis of PCL nanofibers was confirmed by electron dispersive X-ray analysis (EDX). Collagen was immobilized on aminolysed PCL nanofibers using glutaraldehyde as crosslinker. The collagen crosslinking on to PCL nanofibers was established by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The fiber morphologies of PCL nanofibers at different stages were characterized by scanning electron microscopy (SEM). The change in hydrophobicity of PCL nanofibers due to aminolysis and collagen immobilization was determined by water contact angle measurements. Aminolysis followed by collagen immobilization had reduced the intrinsic hydrophobicity of PCL nanofibers. NIH 3T3 fibroblasts were cultured for 2 days on PCL nanofibers, aminolysed PCL nanofibers, and aminolysed PCL nanofibers crosslinked with collagen. Cell attachment and growth were observed by MTT assay in each case. Collagen immobilization improved the biocompatibility of the PCL nanofibers. Thus the modified PCL nanofibers can be used as suitable broad spectrum scaffold for skin, cartilage, bone, cardiac constructs for efficient tissue engineering applications.