Influence of Silicification on the Structural and Biological Properties of Buffer-Mediated Collagen Hydrogels

Authors

  • Sandrine Quignard,

    1. UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 Place Marcelin Berthelot, F-75005 Paris, (France)
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  • Guillermo J. Copello,

    1. IQUIMEFA-CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, 1113 Buenos Aires, (Argentina)
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  • Carole Aimé,

    1. UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 Place Marcelin Berthelot, F-75005 Paris, (France)
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  • Isabelle Bataille,

    1. INSERM, U698, Bioingénierie des Polymères Cardiovasculaires, Institut Galilée, Univ Paris 13, 99 av JB Clément, F-93430 Villetaneuse, (France)
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  • Christophe Hélary,

    1. UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 Place Marcelin Berthelot, F-75005 Paris, (France)
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  • Martin F. Desimone,

    1. IQUIMEFA-CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, 1113 Buenos Aires, (Argentina)
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  • Thibaud Coradin

    Corresponding author
    1. UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 Place Marcelin Berthelot, F-75005 Paris, (France)
    • UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcelin Berthelot, F-75005 Paris, (France).
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  • G. J. C. thanks CONICET for funding his travel to Paris. Funding from the ANR Bone Printing project is acknowledged. We thank Dr. Marie-Madeleine Giraud-Guille and J. Silvent for fruitful discussions and N. Abdoul-Aribi for assistance in chemical analyses. Supporting Information is available from the Wiley Online Library or from the author.

Abstract

A buffer-mediated gelation route for collagen hydrogels that allows the formation of homogeneous composite and hybrid materials with various silica sources (i.e., colloidal silica and soluble silicates) at high concentration (up to 25 × 10−3M) is described. Most significant improvement in rheological properties and proliferation of primary adult human dermal fibroblasts was obtained for the silicate-based hybrid materials. A similar trend was observed in composite materials incorporating 14 nm SiO2 nanoparticles, although to a much lesser extent, whereas larger colloids (80 and 390 nm) did not significantly impact mechanical stability and cell behavior. Modification of 80 nm particles surface with amine groups weakens the collagen-mineral interface, resulting in the decrease of material stability and leading to particle aggregation during the course of cell proliferation experiments.

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