G. J. C. thanks CONICET for funding his travel to Paris. Funding from the ANR Bone Printing project is acknowledged. We thank Dr. Marie-Madeleine Giraud-Guille and J. Silvent for fruitful discussions and N. Abdoul-Aribi for assistance in chemical analyses. Supporting Information is available from the Wiley Online Library or from the author.
Influence of Silicification on the Structural and Biological Properties of Buffer-Mediated Collagen Hydrogels†
Article first published online: 2 NOV 2011
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Engineering Materials
Volume 14, Issue 3, pages B51–B55, March 2012
How to Cite
Quignard, S., Copello, G. J., Aimé, C., Bataille, I., Hélary, C., Desimone, M. F. and Coradin, T. (2012), Influence of Silicification on the Structural and Biological Properties of Buffer-Mediated Collagen Hydrogels. Adv. Eng. Mater., 14: B51–B55. doi: 10.1002/adem.201180063
- Issue published online: 5 MAR 2012
- Article first published online: 2 NOV 2011
- Manuscript Accepted: 1 OCT 2011
- Manuscript Received: 12 JUL 2011
- ANR Bone Printing
A buffer-mediated gelation route for collagen hydrogels that allows the formation of homogeneous composite and hybrid materials with various silica sources (i.e., colloidal silica and soluble silicates) at high concentration (up to 25 × 10−3 M) is described. Most significant improvement in rheological properties and proliferation of primary adult human dermal fibroblasts was obtained for the silicate-based hybrid materials. A similar trend was observed in composite materials incorporating 14 nm SiO2 nanoparticles, although to a much lesser extent, whereas larger colloids (80 and 390 nm) did not significantly impact mechanical stability and cell behavior. Modification of 80 nm particles surface with amine groups weakens the collagen-mineral interface, resulting in the decrease of material stability and leading to particle aggregation during the course of cell proliferation experiments.