Molding Micropatterns of Elasticity on PEG-Based Hydrogels to Control Cell Adhesion and Migration (pages B395–B404)
Mar Diez, Vera A. Schulte, Filippo Stefanoni, Carlo F. Natale, Francesco Mollica, Claudia M. Cesa, Jingyu Chen, Martin Möller, Paolo A. Netti, Maurizio Ventre and Marga C. Lensen
Article first published online: 7 MAR 2011 | DOI: 10.1002/adem.201080122
An innovative and versatile soft lithographic method is presented, which is denoted FIMIC (FIll-Molding In Capillaries). We have employed this method to fabricate smooth, hybrid surfaces with a micropattern of elasticity, using two hydrogel formulations with different crosslinking density. The hydrogels are prepared from PEG-based macromonomers that are liquid before crosslinking, which is an essential requirement for the FIMIC process. The resulting hybrid hydrogels with micropatterns of elasticity represent a very useful platform to study cell motility in dependence of substrate rigidity, e.g. “durotaxis”. The first cell culture results show that the fibroblasts selectively move around on and eventually adhere to the stiffer regions.