Pyridylamino-β-cyclodextrin as a Molecular Chaperone for Lipopolysaccharide Embedded in a Multilayered Polyelectrolyte Architecture

Authors


  • We are grateful for financial support from the Balaton programme. The authors (P. S. and N. B. J.) are indebted to the Faculty of Odontology of Strasbourg for financial support.

Abstract

Layer-by-layer self-assembled polyelectrolyte films containing a charged cyclodextrin and lipopolysaccharide (LPS) are developed for the first time as a potential model for local endotoxin antagonist delivery. We have examined the biological activity of a lipopolysaccharide from E. coli incorporated into multilayered architectures made of poly-(L-lysine) and poly-(L-glutamic acid). Used in such build-ups, a polycationic cyclodextrin, heptakis(6-deoxy-6-pyridylamino)-β-cyclodextrin showed molecular chaperone properties by enabling restoration of the LPS biological activity whenever lost upon interaction with poly-(L-lysine).

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