Pyridylamino-β-cyclodextrin as a Molecular Chaperone for Lipopolysaccharide Embedded in a Multilayered Polyelectrolyte Architecture


  • We are grateful for financial support from the Balaton programme. The authors (P. S. and N. B. J.) are indebted to the Faculty of Odontology of Strasbourg for financial support.


Layer-by-layer self-assembled polyelectrolyte films containing a charged cyclodextrin and lipopolysaccharide (LPS) are developed for the first time as a potential model for local endotoxin antagonist delivery. We have examined the biological activity of a lipopolysaccharide from E. coli incorporated into multilayered architectures made of poly-(L-lysine) and poly-(L-glutamic acid). Used in such build-ups, a polycationic cyclodextrin, heptakis(6-deoxy-6-pyridylamino)-β-cyclodextrin showed molecular chaperone properties by enabling restoration of the LPS biological activity whenever lost upon interaction with poly-(L-lysine).