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Primary Cell Adhesion on RGD-Functionalized and Covalently Crosslinked Thin Polyelectrolyte Multilayer Films

Authors

  • C. Picart,

    1. Ecole Européenne de Chimie, Polymères et Matériaux de Strasbourg, 25 rue Becquerel, F-67085 Strasbourg Cedex 2, France
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  • R. Elkaim,

    1. Parogène, 11 rue Humann, F-67085 Strasbourg Cedex, France
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  • L. Richert,

    1. Institut National de la Santé et de la Recherche Médicale, Unité 595, Université Louis Pasteur, Faculté de Médecine, Bât 3, 11 rue Humann, F-67085 Strasbourg Cedex, France
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  • F. Audoin,

    1. Laboratoire de Chimie Bioorganique, UMR 7514 CNRS-ULP, Faculté de Pharmacie, 74 route du Rhin, F-67400 Illkirch, France
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  • Y. Arntz,

    1. Institut National de la Santé et de la Recherche Médicale, Unité 595, Université Louis Pasteur, Faculté de Médecine, Bât 3, 11 rue Humann, F-67085 Strasbourg Cedex, France
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  • M. Da Silva Cardoso,

    1. Institut National de la Santé et de la Recherche Médicale, Unité 595, Université Louis Pasteur, Faculté de Médecine, Bât 3, 11 rue Humann, F-67085 Strasbourg Cedex, France
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  • P. Schaaf,

    1. Ecole Européenne de Chimie, Polymères et Matériaux de Strasbourg, 25 rue Becquerel, F-67085 Strasbourg Cedex 2, France
    2. Institut Charles Sadron, Centre National de la Recherche Scientifique, Université Louis Pasteur, 6 rue Boussingault, F-67083 Strasbourg Cedex, France
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  • J.-C. Voegel,

    1. Institut National de la Santé et de la Recherche Médicale, Unité 595, Université Louis Pasteur, Faculté de Médecine, Bât 3, 11 rue Humann, F-67085 Strasbourg Cedex, France
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  • B. Frisch

    1. Laboratoire de Chimie Bioorganique, UMR 7514 CNRS-ULP, Faculté de Pharmacie, 74 route du Rhin, F-67400 Illkirch, France
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  • We are grateful to Cosette Betscha for her help with the osteoblasts culture. We thank Fouzia Boulmedais for her help with the FTIR experiments and C. Gergely for fruitful discussions about PLL/PGA films. We are also grateful to Anne-Laure Bolcato-Bellemin for fruitful discussions regarding the biological tests. This work was supported by the programs ACI “Technologies pour la Santé” (2002) from the Ministère Français de la Recherche.

Abstract

Polyelectrolyte multilayers (PEMs) are now widely used for biomedical applications. In this work, we investigated the primary osteoblast adhesion properties of PEMs of poly(L-lysine) (PLL), poly(L-glutamic acid) (PGA), poly(alginic acid) (Palg), and poly(galacturonic acid) (Pgal). In order to compensate for the poor adhesion of the as-synthesized films, two kinds of film modifications were achieved: a purely physical modification by film crosslinking, and a chemical modification by grafting a arginine–glycine–aspartic acid (RGD) peptide to PGA. Crosslinking was performed using a water-soluble carbodiimide in combination with N-hydroxysulfosuccinimide (sulfo-NHS) to induce amide formation. This reaction was followed by Fourier-transform IR spectroscopy. For film functionalization, a 15-amino-acid peptide was grafted to PGA and deposited as the top layer of the film. PLL/PGA, PLL/Palg, and PLL/Pgal films were crosslinked or functionalized. The films were tested for both short-term adhesion properties and long-term proliferation of primary osteoblasts. Whereas the effect of film crosslinking on short-term adhesion was moderate, it was much more important for the RGD-functionalized films. On the other hand, the long-term proliferation was the same or even higher for the crosslinked films as compared with the functionalized films. This effect was particularly enhanced for the PLL/Palg and PLL/Pgal films. Finally, we functionalized PLL/PGA that had been crosslinked prior to PGA-RGD deposition. These architectures exhibited even higher short-term adhesion and proliferation. These results clearly show the important role of the physical properties of the films, besides their chemical properties, for the modulation of primary cell-adhesion behavior.

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