Pseudobilayer Vesicle Formation via Layer-by-Layer Assembly of Hydrophobically Modified Polymers on Sacrificial Substrates


  • We thank Christine Pilz and Anne Heilig for technical assistance. AJK acknowledges the Alexander von Humboldt foundation for a fellowship grant.


A bilayer of a hydrophobically modified polyelectrolyte, octadecyl poly(acrylamide) (PAAm), sandwiched between the layers of a hydrophilic polyelectrolyte, poly(ethyleneimine) (PEI), is prepared by the sequential electrostatic–hydrophobic–electrostatic-interaction-driven self-assembly on planar and colloid substrates. This process results in a PEI/[PAAm]2/PEI-multilayer-coated substrate. The removal of a PAA/PEI/[PAAm]2/PEI-multilayer-coated decomposable colloidal template produces hollow capsules. Irregular hydrophobic domains of the [PAAm]2 bilayer in the PEI/[PAAm]2/PEI-multilayer capsule are infiltrated with a lipid to obtain a uniform, distinct hydrophobic layer, imparting the capsule with a pseudobilayer vesicle structure.