Antibody-Functionalized Hybrid Superparamagnetic Nanoparticles


  • Support from the Spanish Nanoscience Action NAN200409270C3-1/2, Ciber CB06/01/0026 and from the Consolider program, CSD2006-00012 is gratefully acknowledged. We thank Ms. Ana Pérez for her valuable help with the FTIR measurements. One of the authors, M.A., acknowledges support of a contract from the “Juan de la Cierva” program (project PPQ2003-04986). S.I. acknowledges the support of the “Ramón y Cajal” program.


Superparamagnetic hybrid nanoparticles (ca. 80 nm) are obtained. They consist of an inner iron oxide core coated by a silica shell, which is in turn functionalized with amine or carboxyl groups and covalently coupled to a monoclonal antibody (anti-hCG; hCG = human chorionic gonadotropin). The prepared nanoparticles show a specific magnetic moment (per gram of iron) that is comparable to that measured for commercial superparamagnetic iron oxide preparations. The bioactivity of the antibody-conjugated magnetic nanoparticles is verified by a standard bioassay. These results indicate the potential of the hybrid nanoparticles prepared for use as enhanced contrast agents in magnetic resonance imaging applications.