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Antibody-Functionalized Hybrid Superparamagnetic Nanoparticles†

  1. M. Arruebo1,
  2. R. Fernández-Pacheco1,
  3. B. Velasco1,
  4. C. Marquina2,
  5. J. Arbiol3,
  6. S. Irusta1,
  7. M. R. Ibarra1,2,
  8. J. Santamaría1

Article first published online: 30 APR 2007

DOI: 10.1002/adfm.200600560

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 17, Issue 9, pages 1473–1479, June, 2007

Additional Information

How to Cite

Arruebo, M., Fernández-Pacheco, R., Velasco, B., Marquina, C., Arbiol, J., Irusta, S., Ibarra, M. R. and Santamaría, J. (2007), Antibody-Functionalized Hybrid Superparamagnetic Nanoparticles. Advanced Functional Materials, 17: 1473–1479. doi: 10.1002/adfm.200600560

Author Information

  1. 1

    Nanoscience Institute of Aragon (INA), Pedro Cerbuna 12, University of Zaragoza, 50009 Zaragoza (Spain)

  2. 2

    Materials Science Institute of Aragon, CSIC–Zaragoza University, 50009 Zaragoza (Spain)

  3. 3

    TEM-MAT, Serveis Cientificotècnics, Universitat de Barcelona, 08028 Barcelona (Spain)

  1. †

    Support from the Spanish Nanoscience Action NAN200409270C3-1/2, Ciber CB06/01/0026 and from the Consolider program, CSD2006-00012 is gratefully acknowledged. We thank Ms. Ana Pérez for her valuable help with the FTIR measurements. One of the authors, M.A., acknowledges support of a contract from the “Juan de la Cierva” program (project PPQ2003-04986). S.I. acknowledges the support of the “Ramón y Cajal” program.

Publication History

  1. Issue published online: 1 JUN 2007
  2. Article first published online: 30 APR 2007
  3. Manuscript Revised: 4 NOV 2006
  4. Manuscript Received: 26 JUN 2006

Funded by

  • Spanish Nanoscience Action. Grant Number: NAN200409270C3-1/2
  • Consolider program. Grant Number: CSD2006-00012
  • “Juan de la Cierva” program. Grant Number: PPQ2003-04986
  • “Ramón y Cajal” program

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Keywords:

  • Biocompatible materials;
  • Core/shell nanoparticles;
  • Iron oxides;
  • Silicon;
  • Superparamagnetism

Graphical Abstract

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Superparamagnetic hybrid nanoparticles are obtained; they consist of an inner iron oxide core coated by a silica shell (see figure) and are covalently coupled to a monoclonal antibody (anti-hCG). This has been verified by an immunochromatographic assay. These results point to the potential of the hybrid nanoparticles prepared for use as enhanced contrast agents in magnetic resonance imaging applications.

Abstract

Superparamagnetic hybrid nanoparticles (ca. 80 nm) are obtained. They consist of an inner iron oxide core coated by a silica shell, which is in turn functionalized with amine or carboxyl groups and covalently coupled to a monoclonal antibody (anti-hCG; hCG = human chorionic gonadotropin). The prepared nanoparticles show a specific magnetic moment (per gram of iron) that is comparable to that measured for commercial superparamagnetic iron oxide preparations. The bioactivity of the antibody-conjugated magnetic nanoparticles is verified by a standard bioassay. These results indicate the potential of the hybrid nanoparticles prepared for use as enhanced contrast agents in magnetic resonance imaging applications.

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