This project was supported by the National Nature Science Foundation of China under Contract Nos. 20 221 603, 20 536 050, and 20 376 082. The authors wish to thank Xing-Zhu Zhou, Yong-Hong Gu, Qing Xia, and Meng-Yao Li for their assistance. Supporting Information is available online from Wiley InterScience or from the author.
Preparation and Application of Novel Microspheres Possessing Autofluorescent Properties†
Article first published online: 4 OCT 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Functional Materials
Volume 17, Issue 16, pages 3153–3158, November, 2007
How to Cite
Wei, W., Wang, L.-Y., Yuan, L., Wei, Q., Yang, X.-D., Su, Z.-G. and Ma, G.-H. (2007), Preparation and Application of Novel Microspheres Possessing Autofluorescent Properties. Adv. Funct. Mater., 17: 3153–3158. doi: 10.1002/adfm.200700274
- Issue published online: 29 OCT 2007
- Article first published online: 4 OCT 2007
- Manuscript Revised: 30 APR 2007
- Manuscript Received: 7 MAR 2007
- National Nature Science Foundation of China. Grant Numbers: 20 221 603, 20 536 050, 20 376 082
- Fluorescence imaging;
- Polymeric materials
Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The autofluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers.