Financial support from the 973 Program (2006CB806200, China) and the NSFC (20473044, 20533050 and 50673048) are gratefully acknowledged. Supporting information is available online from Wiley InterScience or from the author.
Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels†
Article first published online: 12 FEB 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Functional Materials
Volume 18, Issue 4, pages 575–583, February 22, 2008
How to Cite
Hu, X., Li, G., Li, M., Huang, J., Li, Y., Gao, Y. and Zhang, Y. (2008), Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels. Adv. Funct. Mater., 18: 575–583. doi: 10.1002/adfm.200700527
- Issue published online: 28 FEB 2008
- Article first published online: 12 FEB 2008
- Manuscript Revised: 29 OCT 2007
- Manuscript Received: 13 MAY 2007
- 973 Program China. Grant Number: 2006CB806200
- NSFC. Grant Numbers: 20473044, 20533050, 50673048
Taking theophylline and (1R,2S)-(−)-ephedrine as template molecules, two imprinted photonic-hydrogel films are prepared by a combination of colloidal-crystal and molecular-imprinting techniques. This paper shows a new approach for rapid and handy stimulant detection with high sensitivity and specificity. One film is proposed for analogous molecule assay, another one for chiral recognition. The key point of this approach is that the imprinted photonic polymer (IPP) consists of a three-dimensional (3D), highly-ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. This special, bicontinuous, hierarchical structure enables this polymer to report quickly, easily, sensitively and directly a molecular recognition event without any transducers and treatments for analytes (label-free). The inherent affinity of the nanocavities, deriving from molecular imprinting, makes these sensors highly specific to analytes, even if in a competitive environment. Their sensitive and specific responses to stimulants in buffer are determined by Bragg diffractive shifts due to the lattice change of their 3D ordered macroporous arrays resulting from their preferential rebinding to the target molecules. The measurements show that the prepared hydrogel films exhibit high sensitivity in such a 0.1 fM concentration of analytes and specificity even in a competitive urinous buffer. The reported method provides a rapid and handy approach for stimulant assay and drug analysis in athletic sports.