We are grateful to Dr. André Walder for providing Ti 40 samples (PROTIP) and the SODERN Company (EADS affiliated company) for the sintering of titanium porous parts. We thank Dr. Jean-Hervé Lignot (CNRS-CEPE, Strasbourg, France) for its help with the SEM and Dr. Bernard Senger (INSERM U595, Strasbourg, France) for statistical analyses. This study was supported by Grants from the Réseau National des Technologies de Santé (RNTS) de l'Agence Nationale de la Recherche (ANR, “Protimplant Project”), and from ANR Tecsan (“Subvacel Project”). P.L. is indebted to Hôpitaux Universitaires de Strasbourg for financial support. Supporting Information is available online from Wiley InterScience or from the authors.
VEGF-Functionalized Polyelectrolyte Multilayers as Proangiogenic Prosthetic Coatings†
Article first published online: 6 JUN 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Functional Materials
Volume 18, Issue 12, pages 1767–1775, June 24, 2008
How to Cite
Müller, S., Koenig, G., Charpiot, A., Debry, C., Voegel, J.-C., Lavalle, P. and Vautier, D. (2008), VEGF-Functionalized Polyelectrolyte Multilayers as Proangiogenic Prosthetic Coatings. Adv. Funct. Mater., 18: 1767–1775. doi: 10.1002/adfm.200701233
- Issue published online: 18 JUN 2008
- Article first published online: 6 JUN 2008
- Manuscript Revised: 10 JAN 2008
- Manuscript Received: 25 OCT 2007
- Polyelectrolyte multilayers;
- Porous materials;
- Biocompatible materials
Stimulation of transprosthetic vascularization represents an interesting strategy in implantology to allow rapid tissue integration and finally to avoid prosthetic rejection. To achieve this goal, we modified the surface of porous titanium implants with polyelectrolyte multilayer (PEM) films functionalized with vascular endothelial growth factor (VEGF). Among the two PEM systems investigated, poly(L-lysine)/poly(L-glutamic acid) (PLL/PGA) and poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate) (PAH/PSS), the (PAH/PSS)4 architecture was selected to functionalize porous titanium, both for its high efficiency to adsorb VEGF and for its biocompatibility toward endothelial cells. In an original way, we unambiguously demonstrated that VEGF adsorbed on (PAH/PSS)4 maintains its bioactivity in vitro and stimulates endothelial cells proliferation. This effect was correlated with specific activation of intracellular signaling pathways induced by successive phosphorylation of the endothelial VEGF receptor VEGFR2 and mitogen-activated protein kinases (MAPK) ERK1/2. By clearly demonstrating the proangiogenic activity of the VEGF-PEM coating in vitro, the present study constitutes a first step toward in vivo application.