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Evaluation of Encoded Layer-By-Layer Coated Microparticles As Protease Sensors†

  1. Barbara G. Stubbe1,
  2. Kevin Gevaert2,3,
  3. Stefaan Derveaux1,
  4. Kevin Braeckmans1,
  5. Bruno G. De Geest1,
  6. Marc Goethals2,3,
  7. Joel Vandekerckhove2,3,
  8. Joseph Demeester1,
  9. Stefaan C. De Smedt1,*

Article first published online: 3 JUN 2008

DOI: 10.1002/adfm.200701356

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 18, Issue 11, pages 1624–1631, June 11, 2008

Additional Information

How to Cite

Stubbe, B. G., Gevaert, K., Derveaux, S., Braeckmans, K., De Geest, B. G., Goethals, M., Vandekerckhove, J., Demeester, J. and De Smedt, S. C. (2008), Evaluation of Encoded Layer-By-Layer Coated Microparticles As Protease Sensors. Advanced Functional Materials, 18: 1624–1631. doi: 10.1002/adfm.200701356

Author Information

  1. 1

    Laboratory of Biochemistry and Physical Pharmacy Department of Pharmaceutics, Ghent University 9000 Ghent (Belgium)

  2. 2

    Department of Medical Protein Research, VIB 9000 Ghent (Belgium)

  3. 3

    Department of Biochemistry, Ghent University 9000 Ghent (Belgium)

*Laboratory of Biochemistry and Physical Pharmacy Department of Pharmaceutics, Ghent University 9000 Ghent (Belgium).

  1. †

    Ghent University is thanked for a post-doctoral BOF scholarship to B.S. and for instrumentation credits. Marta Wójtowicz is thanked for practical assistance. We acknowledge the Fund for Scientific Research– Flanders (Belgium), for support by research grants (project numbers G.0156.05, G.0077.06, G.0024.06, and G.0042.07), the Concerted Research Actions (project BOF07/GOA/012) from Ghent University, the Inter University Attraction Poles (IUAP06), and the European Union Interaction Proteome (6th Framework Program. Supporting Information is available online from Wiley InterScience or from the author.

Publication History

  1. Issue published online: 12 JUN 2008
  2. Article first published online: 3 JUN 2008
  3. Manuscript Revised: 15 JAN 2008
  4. Manuscript Received: 21 NOV 2007

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Keywords:

  • biosensors;
  • fluorescence;
  • multilayers;
  • peptides;
  • polyelectrolytes

Graphical Abstract

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Layer-by-layer (LbL) polyelectrolyte coatings, containing a red-labeled trypsin substrate, are carefully designed and applied at the surface of encoded microparticles. The peptide-loaded LbL coatings lose their red fluorescence upon incubation in a trypsin solution, indicating that LbL-coated microparticles show potential to screen for the presence of active proteases in biological samples (see figure).

Abstract

Proteases are important pharmaceutical targets for new drugs because of their involvement in numerous disease processes. This study evaluates whether photophysically encoded microparticles carrying fluorescently labeled protease substrates (peptides) at their surface show potential for detecting proteases in a sample. Layer-by-layer (LbL) polyelectrolyte coatings, containing a red-labeled peptidic trypsin substrate, are carefully designed and applied at the surface of the encoded microparticles. The peptide-loaded LbL coatings lose their red fluorescence upon incubation in a trypsin solution, indicating that LbL-coated microparticles show potential to screen for the presence of active proteases in biological samples.

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