The authors acknowledge the financial support from the Portuguese Foundation for Science and Technology (FCT) and through POCTI and FEDER programs. The funding provided by Canon Foundation in Europe is gratefully acknowledged. This work was also carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT- 2004-500283) and HIPPOCRATES STREP project (NMP3-CT-2003- 505758). Supporting Information is available online at Wiley InterScience or from the author.
Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting†
Article first published online: 10 JUN 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Functional Materials
Volume 18, Issue 12, pages 1840–1853, June 24, 2008
How to Cite
Oliveira, J. M., Kotobuki, N., Marques, A. P., Pirraco, R. P., Benesch, J., Hirose, M., Costa, S. A., Mano, J. F., Ohgushi, H. and Reis, R. L. (2008), Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting. Adv. Funct. Mater., 18: 1840–1853. doi: 10.1002/adfm.200800165
- Issue published online: 18 JUN 2008
- Article first published online: 10 JUN 2008
- Manuscript Received: 31 JAN 2008
- Portuguese Foundation for Science and Technology (FCT)
- FEDER programs
- Canon Foundation in Europe
- drug delivery;
Novel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere-like nanoparticles that are proposed to be used as carriers to deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The nanoparticles did not exhibit significant cytotoxicity in the range of concentrations below 1 mg mL−1, and fluorescent probe labeled nanoparticles were found to be internalized with highly efficiency by both human osteoblast-like cells and rat bone marrow stromal cells, under fluorescence-activated cell sorting and fluorescence microscopy analyses. Dexamethasone (Dex) has been incorporated into CMCht/PAMAM dendrimer nanoparticles and release rates were determined by high performance liquid chromatography. Moreover, the biochemical data demonstrates that the Dex-loaded CMCht/PAMAM dendrimer nanoparticles promote the osteogenic differentiation of rat bone marrow stromal cells, in vitro. The nanoparticles exhibit interesting physicochemical and biological properties and have great potential to be used in fundamental cell biology studies as well as in a variety of biomedical applications, including tissue engineering and regenerative medicine.