Fabrication and Size-Selective Bioseparation of Magnetic Silica Nanospheres with Highly Ordered Periodic Mesostructure

Authors

  • Lei Zhang,

    1. College of Chemistry and Chemical Engineering, China University of Petroleum Dongying 257061 (China)
    2. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
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  • Shizhang Qiao,

    Corresponding author
    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
    • ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia).
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  • Yonggang Jin,

    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
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  • Huagui Yang,

    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
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  • Sandy Budihartono,

    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
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  • Frances Stahr,

    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
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  • Zifeng Yan,

    Corresponding author
    1. College of Chemistry and Chemical Engineering, China University of Petroleum Dongying 257061 (China)
    • College of Chemistry and Chemical Engineering, China University of Petroleum Dongying 257061 (China).
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  • Xiaolin Wang,

    1. Spintronic and Electronic Materials Group, Institute for Superconducting and Electronic Materials, University of Wollongong NSW 2522 (Australia)
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  • Zhengping Hao,

    1. Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences Beijing 100085 (China)
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  • Gao Qing Lu

    Corresponding author
    1. ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia)
    • ARC Centre of Excellence for Functional Nanomaterials, School of Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 (Australia).
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  • This work was financially supported by the Australian Research Council (ARC) through Discovery Project program (DP0452461), UQ Middle Career Fellowship for SZQ and the ARC Centre of Excellence for Functional Nanomaterials. The authors thank Ms. Pei Yuan and Mr. Yanan Guo of Microscopy and Microanalysis Center, University of Queensland for their part works in TEM characterization. Lei Zhang acknowledges a scholarship from the China Scholarship Council (CSC). Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

In this paper, we report a novel synthesis and selective bioseparation of the composite of Fe3O4 magnetic nanocrystals and highly ordered MCM-41 type periodic mesoporous silica nanospheres. Monodisperse superparamagnetic Fe3O4 nanocrystals were synthesized by thermal decomposition of iron stearate in diol in an autoclave at low temperature. The synthesized nanocrystals were encapsulated in mesoporous silica nanospheres through the packing and self-assembly of composite nanocrystal–surfactant micelles and surfactant/silica complex. Different from previous studies, the produced magnetic silica nanospheres (MSNs) possess not only uniform nanosize (90 ∼ 140 nm) but also a highly ordered mesostructure. More importantly, the pore size and the saturation magnetization values can be controlled by using different alkyltrimethylammonium bromide surfactants and changing the amount of Fe3O4 magnetic nanocrystals encapsulated, respectively. Binary adsorption and desorption of proteins cytochrome c (cyt c) and bovine serum albumin (BSA) demonstrate that MSNs are an effective and highly selective adsorbent for proteins with different molecular sizes. Small particle size, high surface area, narrow pore size distribution, and straight pores of MSNs are responsible for the high selective adsorption capacity and fast adsorption rates. High magnetization values and superparamagnetic property of MSNs provide a convenient means to remove nanoparticles from solution and make the re-dispersion in solution quick following the withdrawal of an external magnetic field.

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