This work was supported by research funding from Singapore Ministry of Education through grant R-279-000-205-112 and doctoral scholarship (for D. H. and X. B.) from the National University of Singapore.
An Air-Supported Liquid Crystal System for Real-Time and Label-Free Characterization of Phospholipases and Their Inhibitors†
Article first published online: 22 SEP 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Functional Materials
Volume 18, Issue 19, pages 2938–2945, October 9, 2008
How to Cite
Hartono, D., Bi, X., Yang, K.-L. and Yung, L.-Y. L. (2008), An Air-Supported Liquid Crystal System for Real-Time and Label-Free Characterization of Phospholipases and Their Inhibitors. Adv. Funct. Mater., 18: 2938–2945. doi: 10.1002/adfm.200800424
- Issue published online: 6 OCT 2008
- Article first published online: 22 SEP 2008
- Manuscript Received: 25 MAR 2008
- Singapore Ministry of Education. Grant Number: R-279-000-205-112
- biomedical applications;
- enzyme inhibitor;
- liquid crystals;
A new air-supported liquid crystal (LC) system for analyzing interfacial phenomena that occur based on the molecular interaction between LCs and adsorbed molecules of interest at the aqueous/LC interface is reported. Compared with existing LC-based detection systems, the miniature system reported here requires less sample and involves simpler preparation. Using this system, the enzymatic hydrolysis of various phospholipases such as phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD) are characterized. The hydrolysis of phospholipid monolayers self-assembled at aqueous/LC interface induces an orientational response from the LCs. As a result, an optical signal that reflects the spatial and temporal distribution of phospholipids during the enzymatic reaction can be generated in a real-time manner. When well-known phospholipase inhibitors are introduced together with respective phospholipases, no orientational response of LCs is observed. In the case of inhibitors MJ33 and compound 48/80, cross-inhibitions among phospholipases are also observed. This work demonstrates that the air-supported LC system provides a facile label-free assay for characterizing phospholipase activities and for screening enzyme inhibitors. It could potentially be useful for different high throughput and cost-effective enzyme screening assays.