Streptavidin-Functionalized Three-Dimensional Ordered Nanoporous Silica Film for Highly Efficient Chemiluminescent Immunosensing

Authors

  • Zhanjun Yang,

    1. MOE Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University, Nanjing 210093 (PR China)
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  • Zhuoying Xie,

    1. State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University, Nanjing 210096 (PR China)
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  • Hong Liu,

    1. MOE Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University, Nanjing 210093 (PR China)
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  • Feng Yan,

    Corresponding author
    1. Jiangsu Institute of Cancer Prevention and Cure Nanjing 210009 (PR China)
    • Jiangsu Institute of Cancer Prevention and Cure Nanjing 210009 (PR China).
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  • Huangxian Ju

    Corresponding author
    1. MOE Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University, Nanjing 210093 (PR China)
    • MOE Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University, Nanjing 210093 (PR China).
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  • This research was financially supported by the Outstanding Medical Talents Program (RC2007069) from Department of Health of Jiangsu Province, the National Science Funds for Creative Research Groups (20521503), the Key Program (20535010) and Major Research Plan (90713015) from the National Natural Science Foundation of China, and the Social Development Projects of Jiangsu (BS2006006, BS2006074). Supporting Information is available online from Wiley InterScience or from the author.

Abstract

A novel biofunctionalized three-dimensional ordered nanoporous SiO2 film is designed for construction of chemiluminescent analytical devices. The nanoporous SiO2 film is prepared with self-assembly of polystyrene spheres as a template and 5-nm SiO2 nanoparticles on a glass slide followed by a calcination process. Its functionalization with streptavidin is achieved by using 3-glycidoxypropyltrimethoxysilane as a linker. Based on the high-selectivity recognition of streptavidin to biotin-labeled antibody a novel immunosensor is further constructed for highly efficient chemiluminescent immunoassay. The surface morphologies and fabrication processes of both the biofunctionalized film and the immunosensor are characterized using scanning electron microscopy, atomic-force microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The three-dimensional ordered nanopores have high capacity for loading of streptavidin and antibody and promote the mass transport of immunoreagents for immunoreaction, thus the resulting chemiluminescent immunosensor shows wide dynamic range for fast immunoassay, and good reproducibility and stability. Using carbohydrate antigen 125 (CA 125) as a model, the highly efficient chemiluminescent immunosensing shows a linear range of three orders of magnitude, from 0.5 to 400 U mL−1. This work provides a biofunctionalized porous nanostructure for promising biosensing applications.

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