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Full Paper
Inorganic Drug-Delivery Nanovehicle Conjugated with Cancer-Cell-Specific Ligand
Article first published online: 13 MAR 2009
DOI: 10.1002/adfm.200801127
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Oh, J.-M., Choi, S.-J., Lee, G.-E., Han, S.-H. and Choy, J.-H. (2009), Inorganic Drug-Delivery Nanovehicle Conjugated with Cancer-Cell-Specific Ligand. Adv. Funct. Mater., 19: 1617–1624. doi: 10.1002/adfm.200801127
Publication History
- Issue published online: 18 MAY 2009
- Article first published online: 13 MAR 2009
- Manuscript Received: 1 AUG 2008
Funded by
- Korea government (MEST). Grant Number: R11-2005-008-00000-0
- MKE (Ministry of Knowledge Economy) through the National R & D project for Nano Science and Technology. Grant Numbers: 2005-0980-1, 2005-0546-1
- Ministry of Knowledge Economy. Grant Number: 10030036
Keywords:
- cancer;
- folates;
- hydroxide;
- drug delivery;
- targeting
Abstract
The surface of layered double hydroxide nanoparticles, a potential drug-delivery nanovehicle, is modified with the cancer-cell-specific ligand, folic acid. The surface modification is successfully accomplished through step-by-step coupling reactions with aminopropyltriethoxysilane and 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide. In order to evaluate the cancer-cell targeting effect of folic-acid-grafted layered double hydroxide utilizing fluorescence-related assay, both layered double hydroxide with and without folic acid moiety are labeled with fluorescein 5′-isothiocyanate. The uptake of layered double hydroxide and folic acid conjugated into KB and A549 cells is visualized using fluorescence microscopy and measured by flow cytometry. Both chemical and biological assay results demonstrate that the folic acid molecules are indeed conjugated to the surface of layered double hydroxide and thus the selectivity of nanovehicles to cancer cells overexpressing folate receptors increases. In this study, it is suggested that layered double hydroxide nanoparticles can be used as drug-delivery carriers with a targeting function due to the chemical conjugation with specific ligand.