Functionalized, Swellable Hydrogel Layers as a Platform for Cell Studies

Authors

  • Núria Marí-Buyé,

    1. Grup d'Enginyeria de Materials Institut Químic de Sarrià-Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
    2. Bioengineering Department Institut Químic de Sarri Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
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  • Shannan O'Shaughnessy,

    1. Department of Chemical Engineering Massachusetts Institute of Technology Cambridge, MA 02138 (USA)
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  • Carles Colominas,

    1. Grup d'Enginyeria de Materials Institut Químic de Sarrià-Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
    2. Bioengineering Department Institut Químic de Sarri Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
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  • Carlos E. Semino,

    1. Bioengineering Department Institut Químic de Sarri Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
    2. Center for Biomedical Engineering Massachusetts Institute of Technology Boston, MA 02139 (USA)
    3. Translational Centre for Regenerative Medicine (TRM) Leipzig University, Leipzig 04103 (Germany)
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  • Karen K. Gleason,

    Corresponding author
    1. Department of Chemical Engineering Massachusetts Institute of Technology Cambridge, MA 02138 (USA)
    • Department of Chemical Engineering Massachusetts Institute of Technology Cambridge, MA 02138 (USA).
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  • Salvador Borrós

    Corresponding author
    1. Grup d'Enginyeria de Materials Institut Químic de Sarrià-Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
    2. Bioengineering Department Institut Químic de Sarri Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain)
    • Grup d'Enginyeria de Materials Institut Químic de Sarrià-Universitat Ramon Llull Via Augusta 390, 08017 Barcelona (Spain).
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Abstract

This paper reports the design, synthesis and characterization of thin films as a platform for studying the separate influences of physical and chemical cues of a matrix on the adhesion, growth and final phenotype of cells. Independent control of the physical and chemical properties of functionalized, swellable hydrogel thin films is achieved using initiated chemical vapor deposition (iCVD). The systematic variation in crosslink density is demonstrated to control the swelling ability of the iCVD hydrogel films based on 2-hydroxyethyl methacrylate (HEMA). At the same time, the incorporation of controllable concentrations of the active ester pentafluorophenyl methacrylate (PFM) allows easy immobilization of aminated bioactive motifs, such as bioactive peptides. Initial cell culture results with human umbilical vein endothelial cells (HUVEC) indicate that the strategy of using PFM to immobilize a cell-adhesion peptide motif onto the hydrogel layers promotes proper HUVEC growth and enhances their phenotype.

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