Full Paper

One-Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High-Performance Biosensing of Glucose and Galactose

  1. Yingchun Fu1,
  2. Penghao Li1,
  3. Qingji Xie1,*,
  4. Xiahong Xu2,
  5. Lihong Lei1,
  6. Chao Chen1,
  7. Can Zou1,
  8. Wenfang Deng1,
  9. Shouzhuo Yao1

Article first published online: 14 APR 2009

DOI: 10.1002/adfm.200801576

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 19, Issue 11, pages 1784–1791, June 9, 2009

Additional Information

How to Cite

Fu, Y., Li, P., Xie, Q., Xu, X., Lei, L., Chen, C., Zou, C., Deng, W. and Yao, S. (2009), One-Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High-Performance Biosensing of Glucose and Galactose. Advanced Functional Materials, 19: 1784–1791. doi: 10.1002/adfm.200801576

Author Information

  1. 1

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China)

  2. 2

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University, Changsha, 410082 (P. R. China)

*Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China).

Publication History

  1. Issue published online: 2 JUN 2009
  2. Article first published online: 14 APR 2009
  3. Manuscript Revised: 17 DEC 2008
  4. Manuscript Received: 22 NOV 2008

Funded by

  • National Natural Science Foundation of China. Grant Numbers: 20675029, 20335020, 90713018
  • Foundations of the Ministry of Education (MOE) of China
  • Hunan Provincial Education Department. Grant Numbers: 05K009, 05A036
  • State Key Laboratory of Electroanalytical Chemistry

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (383K)

Keywords:

  • biosensors;
  • enzymes;
  • nanocomposites;
  • nanoparticles: metallic

Graphical Abstract

Thumbnail image of graphical abstract

Polymer–enzyme–metallic nanoparticle bionanocomposites films with a high load of immobilized high-activity enzyme and obvious electrocatalysis/nano-enhancement effects for biosensing of glucose and galatose are designed and prepared by a one-pot chemical pre-synthesis/electropolymerization protocol, and the prepared enzyme electrodes work well in both first and second-generation biosensing modes.

Abstract

New polymer–enzyme–metallic nanoparticle composite films with a high-load and a high-activity of immobilized enzymes and obvious electrocatalysis/nano-enhancement effects for biosensing of glucose and galactose are designed and prepared by a one-pot chemical pre-synthesis/electropolymerization (CPSE) protocol. Dopamine (DA) as a reductant and a monomer, glucose oxidase (GOx) or galactose oxidase (GaOx) as the enzyme, and HAuCl4 or H2PtCl6 as an oxidant to trigger DA polymerization and the source of metallic nanoparticles, are mixed to yield polymeric bionanocomposites (PBNCs), which are then anchored on the electrode by electropolymerization of the remaining DA monomer. The prepared PBNC material has good biocompatibility, a highly uniform dispersion of the nanoparticles with a narrow size distribution, and high load/activity of the immobilized enzymes, as verified by transmission/scanning electron microscopy and electrochemical quartz crystal microbalance. The thus-prepared enzyme electrodes show a largely improved amperometric biosensing performance, e.g., a very high detection sensitivity (99 or 129 µA cm−2 mM−1 for glucose for Pt PBNCs on bare or platinized Au), a sub-micromolar limit of detection for glucose, and an excellent durability, in comparison with those based on conventional procedures. Also, the PBNC-based enzyme electrodes work well in the second-generation biosensing mode. The proposed one-pot CPSE protocol may be extended to the preparation of many other functionalized PBNCs for wide applications.

Get PDF (383K)