Full Paper

A Direct, Multiplex Biosensor Platform for Pathogen Detection Based on Cross-linked Polydiacetylene (PDA) Supramolecules

  1. Cheol Hee Park1,
  2. Jun Pyo Kim1,
  3. Sang Wook Lee1,
  4. Noo Li Jeon3,
  5. Pil J. Yoo2,
  6. Sang Jun Sim1,*

Article first published online: 2 NOV 2009

DOI: 10.1002/adfm.200900393

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 19, Issue 23, pages 3703–3710, December 9, 2009

Additional Information

How to Cite

Park, C. H., Kim, J. P., Lee, S. W., Jeon, N. L., Yoo, P. J. and Sim, S. J. (2009), A Direct, Multiplex Biosensor Platform for Pathogen Detection Based on Cross-linked Polydiacetylene (PDA) Supramolecules. Adv. Funct. Mater., 19: 3703–3710. doi: 10.1002/adfm.200900393

Author Information

  1. 1

    Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea)

  2. 2

    SKKU Advanced Institute of Nanotechnology (SAINT) and School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea)

  3. 3

    Department of Biomedical Engineering University of California Irvine, CA, 92697 (USA)

*Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea).

Publication History

  1. Issue published online: 3 DEC 2009
  2. Article first published online: 2 NOV 2009
  3. Manuscript Revised: 29 JUL 2009
  4. Manuscript Received: 9 MAR 2009

Funded by

  • Korea Science and Engineering Foundation(KOSEF) National Research Laboratory (NRL)
  • Korean government (MEST). Grant Number: R0A-2008-000-20078-0

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Keywords:

  • Polymers;
  • Biosensors;
  • Medical Applications

Abstract

This study focuses on the development of a multiplex pathogen-detection platform based on polydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 mM, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.

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