Full Paper

Lipid-Like Nanoparticles for Small Interfering RNA Delivery to Endothelial Cells

  1. Seung-Woo Cho2,3,
  2. Michael Goldberg4,
  3. Sun Mi Son2,
  4. Qiaobing Xu2,
  5. Fan Yang2,
  6. Ying Mei2,
  7. Said Bogatyrev2,
  8. Robert Langer2,
  9. Daniel G. Anderson1,*

Article first published online: 25 AUG 2009

DOI: 10.1002/adfm.200900519

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 19, Issue 19, pages 3112–3118, October 9, 2009

Additional Information

How to Cite

Cho, S.-W., Goldberg, M., Son, S. M., Xu, Q., Yang, F., Mei, Y., Bogatyrev, S., Langer, R. and Anderson, D. G. (2009), Lipid-Like Nanoparticles for Small Interfering RNA Delivery to Endothelial Cells. Adv. Funct. Mater., 19: 3112–3118. doi: 10.1002/adfm.200900519

Author Information

  1. 1

    David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 45 Carleton Street, E25-342, Cambridge, MA 02142 (USA)

  2. 2

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)

  3. 3

    Department of Anesthesiology Children′s Hospital Boston Harvard Medical School 300 Longwood Avenue, Boston, MA 02115 (USA)

  4. 4

    Department of Chemistry Massachusetts Institute of Technology 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)

*David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 45 Carleton Street, E25-342, Cambridge, MA 02142 (USA).

Publication History

  1. Issue published online: 5 OCT 2009
  2. Article first published online: 25 AUG 2009
  3. Manuscript Received: 19 MAR 2009

Funded by

  • National Institutes of Health. Grant Number: EB000244
  • Alnylam Pharmaceuticals, Inc.

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Keywords:

  • Apoptosis;
  • Endothelial Cells;
  • Ischemia;
  • Lipidoids;
  • siRNA delivery

Abstract

Here, nanoparticles composed of lipid-like materials (lipidoids) to facilitate non-viral delivery of small interfering RNA (siRNA) to endothelial cells (ECs) are developed. Nanoparticles composed of siRNA and lipidoids with small size (∼200 nm) and positive charge (∼34 mV) are formed by self-assembly of lipidoids and siRNA. Ten lipidoids are synthesized and screened for their ability to facilitate the delivery of siRNA into ECs. Particles composed of leading lipidoids show significantly better delivery to ECs than a leading commercially available transfection reagent, Lipofectamine 2000. As a model of potential therapeutic application, nanoparticles composed of the top performing lipidoid, NA114, are studied for their ability to deliver siRNA targeting anti-angiogenic factor (SHP-1) to human ECs. Silencing of SHP-1 expression significantly enhances EC proliferation and decreases EC apoptosis under a simulated ischemic condition.

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