Novel Magnetic Hydroxyapatite Nanoparticles as Non-Viral Vectors for the Glial Cell Line-Derived Neurotrophic Factor Gene

Authors

  • Hsi-Chin Wu,

    1. Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA)
    2. Institute of Biomedical Engineering National Taiwan University No. 1, Sec. 1, Jen-Ai Rd., Jongjheng District Taipei City 100 (Taiwan)
    3. Division of Medical Sciences Visiting Fellows Program Harvard Medical School 260 Longwood Ave. Boston, MA, 02115 (USA)
    Search for more papers by this author
  • Tzu-Wei Wang,

    1. Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA)
    2. Department of Orthopaedic Surgery Brigham and Women's Hospital Harvard Medical School 75 Francis St. Boston, MA, 02115 (USA)
    3. Department of Materials Sciences and Engineering National Tsing Hua University No. 101, Section 2, Kuang-Fu Road Hsinchu 30013 (Taiwan)
    Search for more papers by this author
  • Martha C. Bohn,

    1. Children's Memorial Research Center Northwestern University 2430 N. Halsted Chicago, IL 60614 (USA)
    Search for more papers by this author
  • Feng-Huei Lin,

    Corresponding author
    1. Institute of Biomedical Engineering National Taiwan University No. 1, Sec. 1, Jen-Ai Rd., Jongjheng District Taipei City 100 (Taiwan)
    • Institute of Biomedical Engineering National Taiwan University No. 1, Sec. 1, Jen-Ai Rd., Jongjheng District Taipei City 100 (Taiwan).
    Search for more papers by this author
  • Myron Spector

    Corresponding author
    1. Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA)
    2. Department of Orthopaedic Surgery Brigham and Women's Hospital Harvard Medical School 75 Francis St. Boston, MA, 02115 (USA)
    • Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA).
    Search for more papers by this author

Abstract

Nanoparticles (NPs) of synthetic hydroxyapatite (Hap) and natural bone mineral (NBM) are rendered magnetic by treatment with iron ions using a wet-chemical process. The magnetic NPs (mNPs), which are about 300 nm in diameter, display superparamagnetic properties in a superconducting quantum interference device, with a saturation magnetization of about 30 emu g−1. X-ray diffraction and transmission electron microscopy reveal that the magnetic properties of the NPs are the result of the hetero-epitaxial growth of magnetite on the Hap and NBM crystallites. The mNPs display a high binding affinity for plasmid DNA in contrast to magnetite NPs which do not bind the plasmid well. The mHap and mNBM NPs result in substantial increases in the transfection of rat marrow-derived mesenchymal stem cells with the gene for glial cell line-derived neurotrophic factor (GDNF), with magnetofection compared to transfection in the absence of a magnet. The amount of GDNF recovered in the medium approaches therapeutic levels despite the small amount of plasmid delivered by the NPs.

Ancillary