Porous Polymersomes with Encapsulated Gd-Labeled Dendrimers as Highly Efficient MRI Contrast Agents

Authors

  • Zhiliang Cheng,

    1. Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall Philadelphia, PA 19104 (USA)
    Search for more papers by this author
  • Daniel L. J. Thorek,

    1. Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall Philadelphia, PA 19104 (USA)
    Search for more papers by this author
  • Andrew Tsourkas

    Corresponding author
    1. Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall Philadelphia, PA 19104 (USA)
    • Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall Philadelphia, PA 19104 (USA).
    Search for more papers by this author

Abstract

The use of nanovesicles with encapsulated Gd as magnetic resonance (MR) contrast agents has largely been ignored due to the detrimental effects of the slow water exchange rate through the vesicle bilayer on the relaxivity of encapsulated Gd. Here, the facile synthesis of a composite MR contrast platform is described; it consists of dendrimer conjugates encapsulated in porous polymersomes. These nanoparticles exhibit improved permeability to water flux and a large capacity to store chelated Gd within the aqueous lumen, resulting in enhanced longitudinal relaxivity. The porous polymersomes, ∼130 nm in diameter, are produced through the aqueous assembly of the polymers, polyethylene oxide-b-polybutadiene (PBdEO), and polyethylene oxide-b-polycaprolactone (PEOCL). Subsequent hydrolysis of the caprolactone (CL) block resulted in a highly permeable outer membrane. To prevent the leakage of small Gd-chelate through the pores, Gd was conjugated to polyamidoamine (PAMAM) dendrimers via diethylenetriaminepentaacetic acid dianhydride (DTPA dianhydride) prior to encapsulation. As a result of the slower rotational correlation time of Gd-labeled dendrimers, the porous outer membrane of the nanovesicle, and the high Gd payload, these functional nanoparticles are found to exhibit a relaxivity (R1) of 292 109 mM−1 s−1 per particle. The polymersomes are also found to exhibit unique pharmacokinetics with a circulation half-life of >3.5 h and predominantly renal clearance.

Ancillary