Capsosomes with Multilayered Subcompartments: Assembly and Loading with Hydrophobic Cargo

Authors

  • Leticia Hosta-Rigau,

    1. Institute for Research in Biomedicine Barcelona Science Park CIBER-BBN, Networking Centre on Bioengineering Biomaterials and Nanomedicine Baldiri Reixac 10-12 08028-Barcelona (Spain)
    2. Department of Organic Chemistry University of Barcelona Martí Franqués 1, 08028-Barcelona (Spain)
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  • Brigitte Städler,

    1. Centre for Nanoscience and Nanotechnology (CNST) Department of Chemical and Biomolecular Engineering The University of Melbourne Parkville, Victoria 3010 (Australia)
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  • Yan Yan,

    1. Centre for Nanoscience and Nanotechnology (CNST) Department of Chemical and Biomolecular Engineering The University of Melbourne Parkville, Victoria 3010 (Australia)
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  • Edouard Collins Nice,

    1. Ludwig Institute for Cancer Research Royal Melbourne Hospital PO Box 2008, Parkville, Victoria 3050 (Australia)
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  • Joan K. Heath,

    1. Ludwig Institute for Cancer Research Royal Melbourne Hospital PO Box 2008, Parkville, Victoria 3050 (Australia)
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  • Fernando Albericio,

    1. Institute for Research in Biomedicine Barcelona Science Park CIBER-BBN, Networking Centre on Bioengineering Biomaterials and Nanomedicine Baldiri Reixac 10-12 08028-Barcelona (Spain)
    2. Department of Organic Chemistry University of Barcelona Martí Franqués 1, 08028-Barcelona (Spain)
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  • Frank Caruso

    Corresponding author
    1. Centre for Nanoscience and Nanotechnology (CNST) Department of Chemical and Biomolecular Engineering The University of Melbourne Parkville, Victoria 3010 (Australia)
    • Centre for Nanoscience and Nanotechnology (CNST) Department of Chemical and Biomolecular Engineering The University of Melbourne Parkville, Victoria 3010 (Australia).
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Abstract

Therapeutic artificial cells or organelles are nanoengineered vehicles that are expected to substitute for missing or lost cellular function. The creation of capsosomes, polymer carrier capsules containing liposomal subcompartments, is a promising approach towards constructing such therapeutic devices using the layer-by-layer assembly method. Herein, the assembly of intact, nonaggregated capsosomes containing multiple liposome layers is reported. It is also further demonstrated that thiocoraline, a hydrophobic model peptide with antitumor activity, can be efficiently loaded into the membrane of the liposomal subcompartments of the capsosomes. Cell viability assays verify the activity of the trapped antitumor cargo. It is also shown that pristine capsosomes do not display inherent cytotoxic effects. The ability to tune the number of liposome layers and hence the drug loading in capsosomes as well as their noncytotoxicity provide new opportunities for the creation of therapeutic artificial cells and organelles.

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