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Quantum-Dot-Tagged Bioresponsive Hydrogel Suspension Array for Multiplex Label-Free DNA Detection

  1. Yuanjin Zhao1,
  2. Xiangwei Zhao1,
  3. Baocheng Tang1,
  4. Wenyu Xu1,
  5. Juan Li1,
  6. Jing Hu1,
  7. Zhongze Gu1,2,*

Article first published online: 22 FEB 2010

DOI: 10.1002/adfm.200901812

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 20, Issue 6, pages 976–982, March 24, 2010

Additional Information

How to Cite

Zhao, Y., Zhao, X., Tang, B., Xu, W., Li, J., Hu, J. and Gu, Z. (2010), Quantum-Dot-Tagged Bioresponsive Hydrogel Suspension Array for Multiplex Label-Free DNA Detection. Adv. Funct. Mater., 20: 976–982. doi: 10.1002/adfm.200901812

Author Information

  1. 1

    State Key Laboratory of Bioelectronics Southeast University Nanjing, 210096 (China)

  2. 2

    Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou Suzhou 215123(China)

*State Key Laboratory of Bioelectronics Southeast University Nanjing, 210096 (China).

Publication History

  1. Issue published online: 22 MAR 2010
  2. Article first published online: 22 FEB 2010
  3. Manuscript Revised: 24 NOV 2009
  4. Manuscript Received: 25 SEP 2009

Funded by

  • 333 Talent Project Foundation and Qing Lan Project of Jiangsu Province
  • Jiangsu Science and Technology Department. Grant Numbers: BE 2009148, BE 2008318
  • National Science Foundation of China. Grant Number: 20703010

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Keywords:

  • hydrogels;
  • label-free detection;
  • quantum dots;
  • photonic crystals;
  • multiplex assays

Abstract

A novel hydrogel suspension array, which possesses the joint advantages of quantum-dot-encoded technology, bioresponsive hydrogels, and photonic crystal sensors with full multiplexing label-free DNA detection capability is developed. The microcarriers of the suspension array are quantum-dot-tagged DNA-responsive hydrogel photonic beads. In the case of label-free DNA detection, specific hybridization of target DNA and the crosslinked single-stranded DNA in the hydrogel grid will cause hydrogel shrinking, which can be detected as a corresponding blue shift in the Bragg diffraction peak position of the beads that can be used for quantitatively estimating the amount of target DNA. The results of the label-free DNA detection show that the suspension array has high selectivity and sensitivity with a detection limit of 10−9 M. This method has the potential to provide low cost, miniaturization, and simple and real-time monitoring of hybridization reaction platforms for detecting genetic variations and sequencing genes.

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