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A Reusable Interface Constructed by 3-Aminophenylboronic Acid-Functionalized Multiwalled Carbon Nanotubes for Cell Capture, Release, and Cytosensing

  1. Xue Zhong,
  2. Hai-Jing Bai,
  3. Jing-Juan Xu*,
  4. Hong-Yuan Chen,
  5. Yu-Hua Zhu

Article first published online: 4 MAR 2010

DOI: 10.1002/adfm.200901915

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 20, Issue 6, pages 992–999, March 24, 2010

Additional Information

How to Cite

Zhong, X., Bai, H.-J., Xu, J.-J., Chen, H.-Y. and Zhu, Y.-H. (2010), A Reusable Interface Constructed by 3-Aminophenylboronic Acid-Functionalized Multiwalled Carbon Nanotubes for Cell Capture, Release, and Cytosensing. Adv. Funct. Mater., 20: 992–999. doi: 10.1002/adfm.200901915

Author Information

  1. Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 (P. R. China)

*Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 (P. R. China).

Publication History

  1. Issue published online: 22 MAR 2010
  2. Article first published online: 4 MAR 2010
  3. Manuscript Revised: 12 NOV 2009
  4. Manuscript Received: 11 OCT 2009

Funded by

  • National Natural Science Foundation. Grant Numbers: 20890021, 20775033
  • National Natural Science Funds for Creative Research Groups. Grant Number: 20821063
  • 973 Program. Grant Number: 2007CB936404
  • program for New Century Excellent Talents in University of China

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Keywords:

  • biomedical applications;
  • bionanotechnology;
  • biosensors;
  • carbon nanotubes;
  • stimuli-responsive materials

Abstract

A newly developed electrochemical cell sensor for the determination of K562 leukemia cells using 3-aminophenylboronic acid (APBA)-functionalized multiwalled carbon nanotubes (MWCNTs) films is demonstrated. The films are generated by the covalent coupling between the [BOND]NH2 groups in APBA and the [BOND]COOH group in the acid-oxidized MWCNTs. As a result of the sugar-specific affinity interactions, the K562 leukemia cells are firmly bound to the APBA-functionalized MWCNTs film via boronic acid groups. Compared to electropolymerized APBA films, the presence of MWCNTs not only provides abundant boronic acid domains for cell capture, their high electrical conductivity also makes the film suitable for electrochemical sensing applications. The resulting modified electrodes are tested as cell detection sensors. This work presents a promising platform for effective cell capture and constructing reusable cytosensors.

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