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Enhanced Fluorescence Microscopic Imaging by Plasmonic Nanostructures: From a 1D Grating to a 2D Nanohole Array

  1. Xiaoqiang Cui1,
  2. Keiko Tawa1,*,
  3. Kenji Kintaka1,
  4. Junji Nishii2

Article first published online: 25 FEB 2010

DOI: 10.1002/adfm.200901993

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 20, Issue 6, pages 945–950, March 24, 2010

Additional Information

How to Cite

Cui, X., Tawa, K., Kintaka, K. and Nishii, J. (2010), Enhanced Fluorescence Microscopic Imaging by Plasmonic Nanostructures: From a 1D Grating to a 2D Nanohole Array. Adv. Funct. Mater., 20: 945–950. doi: 10.1002/adfm.200901993

Author Information

  1. 1

    Research Institute for Cell Engineering National Institute of Advanced Industrial Science and Technology (AIST) Ikeda, Osaka 563-8577 (Japan)

  2. 2

    Research Institute for Electronic Science Hokkaido University Kita-ku, Sapporo 001-0021 (Japan)

*Research Institute for Cell Engineering National Institute of Advanced Industrial Science and Technology (AIST) Ikeda, Osaka 563-8577 (Japan).

Publication History

  1. Issue published online: 22 MAR 2010
  2. Article first published online: 25 FEB 2010
  3. Manuscript Revised: 1 DEC 2009
  4. Manuscript Received: 23 OCT 2009

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Keywords:

  • fluorescence;
  • microscopy;
  • nanostructures;
  • plasmon coupling;
  • plasmonics

Abstract

A two-dimensional (2D) plasmonic coupling nanostructure for enhanced fluorescence observation using a microscope is presented. The substrate contained periodically assembled nanohole arrays with a pitch of 400 nm and a depth of 25 nm. In comparison with one-dimensional (1D) gratings, this new substrate presented an excellent surface plasmon coupling ability to illuminate light from all directions. Under an optical microscope, an enhancement in the fluorescence intensity of up to 100 times compared with a plain glass slide was observed. The ability to markedly increase the fluorescence intensity means this technique has great potential for application in biodiagnostics, imaging, sensing, and photovoltaic cells.

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