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A Three-Dimensional and Sensitive Bioassay Based on Nanostructured Quartz Combined with Viral Nanoparticles

Authors

  • Jong-Hwan Lee,

    1. Department of Chemical and Biological Engineering, College of Engineering Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Jung Suk Kim,

    1. Department of Biomedical Engineering, College of Medicine Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Jin-Seung Park,

    1. Department of Chemical and Biological Engineering, College of Engineering Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Wonbae Lee,

    1. Department of Biomedical Engineering, College of Health Science Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Kyung Eun Lee,

    1. School of Life Sciences and Biotechnology Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Sung-Sik Han,

    1. School of Life Sciences and Biotechnology Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Kyu Back Lee,

    Corresponding author
    1. Department of Biomedical Engineering, College of Health Science Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
    • Department of Biomedical Engineering, College of Health Science Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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  • Jeewon Lee

    Corresponding author
    1. Department of Chemical and Biological Engineering, College of Engineering Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
    • Department of Chemical and Biological Engineering, College of Engineering Korea University Anam-dong 5-1, Seoul 136-713 (Republic of Korea)
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Abstract

An effective mask-free method for fabricating high-aspect-ratio pillarlike nanostructures over a large area of a quartz surface via a simple O2 and CF4 two-step reactive ion etching (RIE) procedure is developed. The nanostructured quartz surfaces are successfully combined with the engineered viral particles derived from hepatitis B virus capsid, yielding a novel 3D assay system with attomolar sensitivity, which has great potential for use in sensitive and early detection of various disease markers.

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