Cell Migration: Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy (Adv. Funct. Mater. 10/2009)

Authors

  • Deok-Ho Kim,

    1. Department of Biomedical Engineering Johns Hopkins University Baltimore, MD 21218 (USA)
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  • Chang-Ho Seo,

    1. School of Mechanical and Aerospace Engineering and the Institute of Bioengineering Seoul National University Seoul 151-742 (Korea)
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  • Karam Han,

    1. Department of Biomedical Engineering Johns Hopkins University Baltimore, MD 21218 (USA)
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  • Keon Woo Kwon,

    1. School of Mechanical and Aerospace Engineering and the Institute of Bioengineering Seoul National University Seoul 151-742 (Korea)
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  • Andre Levchenko,

    Corresponding author
    1. Department of Biomedical Engineering Johns Hopkins University Baltimore, MD 21218 (USA)
    • Department of Biomedical Engineering Johns Hopkins University Baltimore, MD 21218 (USA).
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  • Kahp-Yang Suh

    Corresponding author
    1. School of Mechanical and Aerospace Engineering and the Institute of Bioengineering Seoul National University Seoul 151-742 (Korea)
    • School of Mechanical and Aerospace Engineering and the Institute of Bioengineering Seoul National University Seoul 151-742 (Korea).
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Abstract

original image

A novel microtextured cell substrate with variable local density and anisotropy as a platform for guided cell migration is presented by A. Levchenko, K.-Y. Suh, et al. on page 1579. A simple, scalable, and cost-effective technique, capillary force lithography, is used to fabricate precise microtopographic features on an optically transparent glass coverslip. Live cell motility is found to be extremely sensitive to variation in the local density and anisotropy of rectangular lattices, with cell elongation and speed decreasing on a symmetric lattice. Cells integrate orthogonal contact guidance cues when determining the direction of their orientation and movement.

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