Stable Encapsulation of Quantum Dot Barcodes with Silica Shells

Authors

  • Shang-Hsiu Hu,

    1. Department of Bioengineering, University of Washington, Seattle, WA 98195 (USA)
    2. Department of Materials Sciences and Engineering, National Chiao Tung University, Hsinchu, 300 (Taiwan)
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  • Xiaohu Gao

    Corresponding author
    1. Department of Bioengineering, University of Washington, Seattle, WA 98195 (USA)
    • Department of Bioengineering, University of Washington, Seattle, WA 98195 (USA).
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Abstract

Quantum dot-doped mesoporous microbeads (QDMMs) are encapsulated with silica shells for enhanced chemical stability. The results show that a microemulsion procedure is highly efficient in coating QDMMs with polyvinyl alcohol (PVA), which is important in the subsequent deposition of a silica shell. Incorporation of fluorescent silane precursors allows direct observation of silica shells by fluorescence microscopy. The resulting silica-coated QDMMs (QDMM@SiO2) exhibit remarkable stability against solvent-induced QD leaching and chemical-induced fluorescence quenching compared with the uncoated QDMMs. Further development of this technology such as optimization of silica shell thickness, surface modification with non-fouling polymers, and conjugation with biomolecular probes will enable clinical translation of the optical barcoding technology for highly multiplexed detection and screening of genes and proteins.

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