Water-dispersed all-in-one nanoprobes composed of densely integrated peroxyoxalate fuel and a cyanine dye are formulated to optimize the nanoscopic chemiluminescence reaction. It is demonstrated that the chemiluminescent nanoformulation can generate bright near-infrared signal in response to external hydrogen peroxide that is biologically implicated with cell signaling and diseases. Successful imaging of endogenously overproduces hydrogen peroxide and indirect determination of glucose level in vivo with the chemiluminescent nanoprobes offers an opportunity for early diagnosis of diseases.
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