Selective Artificial Receptors Based on Micropatterned Surface-Imprinted Polymers for Label-Free Detection of Proteins by SPR Imaging

Authors

  • Gergely Lautner,

    1. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, H-1111, Hungary
    Search for more papers by this author
  • Jevgeni Kaev,

    1. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, Tallinn, 19086, Estonia
    Search for more papers by this author
  • Jekaterina Reut,

    1. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, Tallinn, 19086, Estonia
    Search for more papers by this author
  • Andres Öpik,

    1. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, Tallinn, 19086, Estonia
    Search for more papers by this author
  • Jörg Rappich,

    1. Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Institut für Si-Photovoltaik, Kekuléstrasse 5, 12489 Berlin, Germany
    Search for more papers by this author
  • Vitali Syritski,

    Corresponding author
    1. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, Tallinn, 19086, Estonia
    • Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, H-1111, Hungary.
    Search for more papers by this author
  • Róbert E. Gyurcsányi

    Corresponding author
    1. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, H-1111, Hungary
    • Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, H-1111, Hungary.
    Search for more papers by this author

Abstract

A novel concept to generate micropatterned surface-imprinted polymers (SIPs) for protein recognition by using standard photolithographic technology is introduced. Avidin-imprinted poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) conducting polymer microbands are prepared directly on surface plasmon resonance (SPR) chips, which enable convenient label-free monitoring of the binding events. The novel surface-imprinted microstructures bind avidin, the template protein, with dissociation constants in the submicromolar range (125 nM). The SIPs have an avidin binding capacity approximately one order of magnitude higher than the corresponding nonimprinted polymers and are able to discriminate among functional homologues of avidin, i.e., neutravidin, extravidin, and streptavidin.

Ancillary