An error occurred in the sequence of the antisense oligonucleotide shown in the supporting information file of this manuscript. New, corrected supporting information can now be found on its Wiley InterScience abstract page.
Information was also missing from the caption of Figure 5; the corrected caption is as follows:
Figure 5. a) Flow cytometry experiment showing the fluorescence intensity of PC-3 cells incubated with Cy3-labeled AON (400 nM) free, complexed to G5 PAMAM or entrapped in plain or Fab′-PEG115-b-P(PrMA28-co-MAA53)/G5 PAMAM PICMs (N/(P + COO–) molar ratio of 1.5). b) Confocal microscopy images of PC-3 cells after treatment with Cy3-AON (red, Panel ii) complexed to A) G5 PAMAM or B) Fab′-PEG115-b-P(PrMA28-co-MAA53)/G5 PAMAM PICMs. Endosomes/lysosomes were stained by LysoTracker (green, Panel i), and the images were merged in Panel iii. c) Bcl-2 gene silencing in PC-3 cells transfected for 5 h with AON (400 nM) or siRNA (25 nM) complexed to G5 PAMAM or entrapped in plain or Fab′-PEG115-b-P(PrMA28-co-MAA53)/G5 or G3 PAMAM PICMs at N/(P + COO–) of 1.5 (final volume of the transfection medium is 1 mL). Control cells were treated with medium alone. The effect of preincubation with 10% FBS, free CD71 or bafilomycin is indicated on the graph. The inset is representative of immunoreactive Bcl-2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as determined by immunoblotting. The molar ratio of the Fab′-PEG145-b-P(PrMA27-co-MAA58) versus PEG115-b-P(PrMA28-co-MAA53) is 2% for all the experiments. The results are representative of several independent experiments. The first 3 lanes on the left hand side of the inset represent the control-untreated cells.