Get access

Labeling and Tracking P2 Purinergic Receptors in Living Cells Using ATP-Conjugated Quantum Dots

Authors

  • Shan Jiang,

    1. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457
    Search for more papers by this author
  • Aiping Liu,

    1. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457
    Search for more papers by this author
  • Hongwei Duan,

    Corresponding author
    1. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457
    • Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
    Search for more papers by this author
  • Jianchow Soo,

    1. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457
    Search for more papers by this author
  • Peng Chen

    Corresponding author
    1. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457
    • Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
    Search for more papers by this author

Abstract

It has been increasingly recognized that adenosine-5′-triphosphate (ATP) signaling through P2 purinergic receptors has a diverse influence on cell functions and human health. In this contribution, a simple and non-invasive method to specifically label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular trafficking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal reflection fluorescence microscopy.

Ancillary