Organic crystals have great potential for the applications in laser devices. This article presents an effective approach for fabrication of distributed feedback single crystal lasers. With the laser interference ablation method, high quality grating structures have been fabricated on the organic single-crystalline thin film materials. The relationship between the depth, periodicity, and laser fluence is discussed. The optical properties, such as photoluminescence, and diffractive properties are studied in detail. With the appropriate period, strong laser emission has been observed from these devices. Distributed feedback lasing is demonstrated from the laser interference ablated organic single crystals for the first time.