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Combination of Graphene Oxide and Thiol-Activated DNA Metallization for Sensitive Fluorescence Turn-On Detection of Cysteine and Their Use for Logic Gate Operations

Authors

  • Youhui Lin,

    1. Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
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  • Yu Tao,

    1. Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
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  • Fang Pu,

    1. Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
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  • Jinsong Ren,

    Corresponding author
    1. Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
    • Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China.
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  • Xiaogang Qu

    Corresponding author
    1. Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
    • Laboratory of Chemical Biology and State Key Laboratory of Rare Earth, Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China.
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Abstract

In this work, a unique, highly sensitive and selective fluorescence turn-on approach for cysteine detection using an ensemble of graphene oxide (GO) and metallized DNA is reported. The method is based on the extraordinarily high quenching efficiency of GO and the specific interaction between cysteine and metallized DNA via robust Ag–S bonds. In the presence of GO, the dye-labeled single-stranded DNA shows weak fluorescence, while it exhibits a dramatic fluorescence increase upon the formation of the double helix through the “activated” metallized DNA by cysteine. In addition, the protocol shows excellent selectivity for cysteine over various other amino acids found in proteins. Importantly, by exploring GO–DNA interactions and the thiol-mediated DNA hybridization, our sensing system can also be utilized to design the “OR” and “INHIBIT” logic gates using cysteine and DNA as inputs. To the author's knowledge, this method is the first example of combining GO and DNA metallization to fabricate a turn-on fluorescent sensor for cysteine and logic gates.

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