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Biofabricating Multifunctional Soft Matter with Enzymes and Stimuli-Responsive Materials

  1. Yi Liu1,
  2. Jessica L. Terrell2,
  3. Chen-Yu Tsao2,
  4. Hsuan-Chen Wu2,
  5. Vishal Javvaji3,
  6. Eunkyoung Kim1,
  7. Yi Cheng4,
  8. Yifeng Wang5,
  9. Rein V. Ulijn6,
  10. Srinivasa R. Raghavan3,
  11. Gary W. Rubloff4,
  12. William E. Bentley1,2,
  13. Gregory F. Payne1,2,*

Article first published online: 19 APR 2012

DOI: 10.1002/adfm.201200095

Issue

Advanced Functional Materials

Advanced Functional Materials

Volume 22, Issue 14, pages 3004–3012, July 24, 2012

Additional Information

How to Cite

Liu, Y., Terrell, J. L., Tsao, C.-Y., Wu, H.-C., Javvaji, V., Kim, E., Cheng, Y., Wang, Y., Ulijn, R. V., Raghavan, S. R., Rubloff, G. W., Bentley, W. E. and Payne, G. F. (2012), Biofabricating Multifunctional Soft Matter with Enzymes and Stimuli-Responsive Materials. Adv. Funct. Mater., 22: 3004–3012. doi: 10.1002/adfm.201200095

Author Information

  1. 1

    Institute for Bioscience and Biotechnology Research, University of Maryland, 5115 Plant Sciences Building, College Park, MD 20742, USA

  2. 2

    Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA

  3. 3

    Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA

  4. 4

    Institute for Systems Research and, Department of Materials Science and Engineering, University of Maryland, College Park, MD 20742, USA

  5. 5

    School of Material Science and Engineering, Wuhan University of Technology, Wuhan, 430070, P. R. China

  6. 6

    WestCHEM Department of Pure and Applied Chemistry, The University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, Scotland, UK

*Institute for Bioscience and Biotechnology Research, University of Maryland, 5115 Plant Sciences Building, College Park, MD 20742, USA.

Publication History

  1. Issue published online: 11 JUL 2012
  2. Article first published online: 19 APR 2012
  3. Manuscript Revised: 19 MAR 2012
  4. Manuscript Received: 11 JAN 2012

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Keywords:

  • peptides;
  • gelatin;
  • microbial transglutaminase;
  • quorum sensing;
  • stimuli-responsive materials

Abstract

Methods that allow soft matter to be fabricated with controlled structure and function would be beneficial for applications ranging from flexible electronics to regenerative medicine. Here, the assembly of a multifunctional gelatin matrix is demonstrated by triggering its self-assembly and then enzymatically assembling biological functionality. Triggered self-assembly relies on electrodeposition of the pH-responsive hydrogelator, 9-fluorenylmethoxycarbonyl-phenylalanine (Fmoc-Phe), in response to electrical inputs that generate a localized pH-gradient. Warm solutions of Fmoc-Phe and gelatin are co-deposited and, after cooling to room temperature, a physical gelatin network forms. Enzymatic assembly employs the cofactor-independent enzyme microbial transglutaminase (mTG) to perform two functions: crosslink the gelatin matrix to generate a thermally stable chemical gel and conjugate proteins to the matrix. To conjugate globular proteins to gelatin these proteins are engineered to have short lysine-rich or glutamine-rich fusion tags to provide accessible residues for mTG-catalysis. Viable bacteria can be co-deposited and entrapped within the crosslinked gelatin matrix and can proliferate upon subsequent incubation. These results demonstrate the potential for enlisting biological materials and mechanisms to biofabricate multifunctional soft matter.

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