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Gold Functionalized Mesoporous Silica Nanoparticle Mediated Protein and DNA Codelivery to Plant Cells Via the Biolistic Method

Authors

  • Susana Martin-Ortigosa,

    1. Center for Plant Transformation, Plant Sciences Institute, Department of Agronomy, Iowa State University, Ames, IA 50011, USA
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  • Justin S. Valenstein,

    1. Department of Chemistry U.S. Department of Energy Ames Laboratory Iowa State University, Ames, IA 50011, USA
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  • Victor S.-Y. Lin,

    1. Department of Chemistry U.S. Department of Energy Ames Laboratory Iowa State University, Ames, IA 50011, USA
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  • Brian G. Trewyn,

    Corresponding author
    1. Department of Chemistry U.S. Department of Energy Ames Laboratory Iowa State University, Ames, IA 50011, USA
    • Department of Chemistry U.S. Department of Energy Ames Laboratory Iowa State University, Ames, IA 50011, USA
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  • Kan Wang

    Corresponding author
    1. Center for Plant Transformation, Plant Sciences Institute, Department of Agronomy, Iowa State University, Ames, IA 50011, USA
    • Center for Plant Transformation, Plant Sciences Institute, Department of Agronomy, Iowa State University, Ames, IA 50011, USA.
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  • This article is dedicated to the memory of Professor Victor S.-Y. Lin, deceased May 4, 2010, in recognition of his inspiration and friendship

Abstract

The synthesis and characterization of a gold nanoparticle functionalized mesoporous silica nanoparticle (Au-MSN) platform for codelivery of proteins and plasmid DNA to plant tissues using a biolistic particle delivery system is reported. The in vitro uptake and release profiles of fluorescently labeled bovine serum albumin (BSA) and enhanced green fluorescent protein (eGFP) are investigated. As a proof-of-concept demonstration, Au-MSN with large average pore diameters (10 nm) are shown to deliver and subsequently release proteins and plasmid DNA to the same cell after passing through the plant cell wall upon bombardment. Release of fluorescent eGFP indicates the delivery of active, non-denatured proteins to plant cells. This advance represents the first example of biolistic-mediated codelivery of proteins and plasmid DNA to plant cells via gold-functionalized MSN and provides a powerful tool for both fundamental and applied research of plant sciences.

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