Electrogenerated Chemiluminescence from a Phenyleneethynylene Derivative and its Ultrasensitive Immunosensing Application Using a Nanotubular Mesoporous Pt–Ag Alloy for Signal Amplification

Authors

  • Mei Yan,

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author
  • Lei Ge,

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author
  • Weiqiang Gao,

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author
  • Jinghua Yu,

    Corresponding author
    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    • Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China.
    Search for more papers by this author
  • Xianrang Song,

    1. Cancer Research Center, Shandong Tumor Hospital, 250117, P. R. China
    Search for more papers by this author
  • Shenguang Ge,

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author
  • Zhiyong Jia,

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author
  • Chengchao Chu

    1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, P. R. China
    Search for more papers by this author

Abstract

Electrogenerated chemiluminescence (ECL) of a novel phenyleneethynylene derivatives [4,4’-(2,5-dimethoxy-1,4-phenylene)bis(ethyne-2,1-diyl) dibenzoic acid; P-acid] possessing carboxylic acid groups at the para positions in aqueous solutions and its first immunosensing application are studied. Nanotubular mesoporous Pt–Ag alloy nanoparticles are first used to fabricate the ECL immunosensor as a signal amplification carrier. Absorption, photoluminescence, cyclic voltammetry, scanning electron microscopy, transmission electron microscopy, and electrochemical impedance spectroscopy are used as powerful tools to characterize P-acid, Pt–Ag alloy nanoparticles, and the fabrication process of the immunosensor. P-acid exhibits two ECL peaks at –1.07 (ECL-1) and 0.64 (ECL-2) V in air-saturated pH 7.4, 0.1 M PBS containing 0.1 M KCl during the cyclic sweep between 1.51 and –2.67 V at 0.1 V s−1; the ECL mechanism of these two ECL peaks is demonstrated. The electron-transfer reaction between electrochemically oxidized P-acid and tri-n-propylamine (TPrA) radical cation is also demonstrated to produce high ECL emissions and used to develop an ultrasensitive ECL immunosensor. In addition, a Pt–Ag alloy nanoparticles amplification carrier for protein ECL analysis is applied for improvement of the detection sensitivity. Thus, this ECL immunosensor exhibits high sensitivity, good reproducibility, rapid response, and long-term stability.

Ancillary