Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4+ T Lymphocytes

Authors

  • Seung-Hyun Jun,

    1. Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
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  • Kwanghee Kim,

    1. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea
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  • Hyo Jin An,

    1. Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
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  • Byoung Chan Kim,

    1. Environment Sensor System Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea
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  • Chung Hee Sonn,

    1. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea
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  • Miju Kim,

    1. Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
    2. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
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  • Junsang Doh,

    1. Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
    2. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
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  • Cassian Yee,

    1. Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 USA
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  • Kyung-Mi Lee,

    Corresponding author
    1. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea
    • Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea.
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  • Jungbae Kim

    Corresponding author
    1. Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
    • Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
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Abstract

A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4+ T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4+ T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4+ T cells for the purpose of cell therapies.

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