Hierachically Structured Hollow Silica Spheres for High Efficiency Immobilization of Enzymes

Authors

  • Shunsheng Cao,

    Corresponding author
    1. School of Materials Science and Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, P. R. China
    2. Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
    • School of Materials Science and Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, P. R. China
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  • Long Fang,

    1. School of Materials Science and Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, P. R. China
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  • Zhiyuan Zhao,

    1. School of Materials Science and Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, P. R. China
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  • Yi Ge,

    Corresponding author
    1. Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
    • Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK.
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  • Sergey Piletsky,

    1. Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
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  • Anthony P. F. Turner

    Corresponding author
    1. Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
    2. Biosensors & Bioelectronics Centre, IFM, Linköping University, SE-58183, Linköping, Sweden
    • Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK.
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Abstract

In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell-in-shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis.

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