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Hot Spot-Localized Artificial Antibodies for Label-Free Plasmonic Biosensing

Authors

  • Abdennour Abbas,

    1. Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St Louis, MO 63130, USA
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  • Limei Tian,

    1. Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St Louis, MO 63130, USA
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  • Jeremiah J. Morrissey,

    1. Department of Anesthesiology, Division of Clinical and Translational Research, Washington University in St. Louis, St Louis, MO 63110, USA
    2. Siteman Cancer Center, 660 S. Euclid Ave., St Louis, MO 63110, USA
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  • Evan D. Kharasch,

    1. Department of Anesthesiology, Division of Clinical and Translational Research, Washington University in St. Louis, St Louis, MO 63110, USA
    2. Siteman Cancer Center, 660 S. Euclid Ave., St Louis, MO 63110, USA
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  • Srikanth Singamaneni

    Corresponding author
    1. Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St Louis, MO 63130, USA
    2. Siteman Cancer Center, 660 S. Euclid Ave., St Louis, MO 63110, USA
    • Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St Louis, MO 63130, USA.
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Abstract

The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein, a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane copolymerization is described. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. It is expected that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.

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