Cationic Polyfluorenes with Phosphorescent Iridium(III) Complexes for Time-Resolved Luminescent Biosensing and Fluorescence Lifetime Imaging

Authors

  • Huifang Shi,

    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
    2. Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore (NUS) 117576, Singapore
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  • Huibin Sun,

    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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  • Huiran Yang,

    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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  • Shujuan Liu,

    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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  • Gareth Jenkins,

    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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  • Wei Feng,

    1. Department of Chemistry, Fudan University, Shanghai, 200433, China
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  • Fuyou Li,

    1. Department of Chemistry, Fudan University, Shanghai, 200433, China
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  • Qiang Zhao,

    Corresponding author
    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
    • Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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  • Bin Liu,

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore (NUS) 117576, Singapore
    • Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore (NUS) 117576, Singapore.
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  • Wei Huang

    Corresponding author
    1. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
    • Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu-Singapore Joint Research Center for Organic/Bio Electronics & Information Displays, and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Wenyuan Road 9, Nanjing City, Jiangsu Province, 210046, China
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Errata

This article is corrected by:

  1. Errata: Polyfluorenes with Phosphorescent Iridium(III) Complexes for Time-Resolved Luminescent Biosensing and Fluorescence Lifetime Imaging Volume 26, Issue 36, 6505, Article first published online: 26 September 2016

Abstract

The application of a time-resolved photoluminescence technique and fluorescence lifetime imaging microscopy for biosensing and bioimaging based on phosphorescent conjugated polyelectrolytes (PCPEs) containing Ir(III) complexes and polyfluorene units is reported. The specially designed PCPEs form 50 nm nanoparticles with blue fluorescence in aqueous solutions. Electrostatic interaction between the nanoparticles and heparin improves the energy transfer between the polyfluorene units to Ir(III) complex, which lights up the red signal for naked-eye sensing. Good selectivity has been demonstrated for heparin sensing in aqueous solution and serum with quantification ranges of 0–70 μM and 0–5 μM, respectively. The signal-to-noise ratio can be further improved through time-resolved emission spectra, especially when the detection is conducted in complicated environment, e.g., in the presence of fluorescent dyes. In addition to heparin sensing, the PCPEs have also been used for specific labeling of live KB cell membrane with high contrast using both confocal fluorescent cellular imaging and fluorescence lifetime imaging microscopies. This study provides a new perspective for designing promising CPEs for biosensing and bioimaging applications.

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