• multiphoton grafting;
  • patterning;
  • photografting;
  • aromatic azides;
  • click chemistry
Thumbnail image of graphical abstract

This 180 μm wide, 3D pattern is produced by laser-induced immobilization of fluorescent molecules onto a polymeric matrix. On page 3429 Aleksandr Ovsianikov, Robert Liska, and co-workers report a versatile and straightforward photografting method based on photolysis of an aromatic azide via multiphoton absorption. Because multiphoton-induced reactions occur in a confined area within the laser focal spot, accurate functionalization with high spatial resolution in 3D is possible. The presented results indicate the great potential of the 3D, site-specific, function-alization method for applications in microarray-based proteome analysis, studies of cell–surface interactions, sensing applications, and drug screening.