Catalytic Saloplastics: Alkaline Phosphatase Immobilized and Stabilized in Compacted Polyelectrolyte Complexes

Authors

  • Patricia Tirado,

    1. Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France
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  • Andreas Reisch,

    1. Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France
    Current affiliation:
    1. CNRS UMR 7213, Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch CEDEX, France
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  • Emilie Roger,

    1. Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France
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  • Fouzia Boulmedais,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France
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  • Loïc Jierry,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France
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  • Philippe Lavalle,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Université de Strasbourg, Faculté de Chirurgie Dentaire, 67000 Strasbourg, France
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  • Jean-Claude Voegel,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Université de Strasbourg, Faculté de Chirurgie Dentaire, 67000 Strasbourg, France
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  • Pierre Schaaf,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France
    2. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Université de Strasbourg, Faculté de Chirurgie Dentaire, 67000 Strasbourg, France
    3. International Center for Frontier Research in Chemistry, 67000 Strasbourg, France
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  • Joseph B. Schlenoff,

    1. Department of Chemistry and Biochemistry, The Florida State University, Tallahassee, FL 32306, USA
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  • Benoît Frisch

    Corresponding author
    1. Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France
    2. International Center for Frontier Research in Chemistry, 67000 Strasbourg, France
    • Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199, CNRS/Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France.
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Abstract

Novel biochemically active compact polyelectrolyte complexes (CoPECs) are obtained through a simple coprecipitation and compaction procedure. As shown for the system composed of poly(acrylic acid) (PAA) and poly(allylamine) (PAH) as polyelectrolytes and alkaline phosphatase (ALP) as enzyme, the enzyme can be firmly immobilized into these materials. The ALP not only remains active in these materials, but the matrix also enhances the specific activity of the enzyme while protecting it from deactivation at higher temperature. The presence of the matrix allows fine control and substantial enhancement of reaction rates by varying the salt concentration of the contacting solution or temperature. The excellent reusability, together with the ease of co-immobilizing other useful components, such as magnetic particles, allowing facile handling of the CoPECs, makes these materials interesting candidates for variable scaffolds for the immobilization of enzymes for small- and large-scale enzyme-catalyzed processes.

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