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Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide

Authors

  • G. Cado,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
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  • R. Aslam,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • L. Séon,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
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  • T. Garnier,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
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  • R. Fabre,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • A. Parat,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
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  • A. Chassepot,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • J.-C. Voegel,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • B. Senger,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • F. Schneider,

    1. Service de Réanimation Médicale, Hôpital de Hautepierre, Hôpitaux Universitaires de Strasbourg, 1 avenue Molière, 67098 Strasbourg Cedex, France
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  • Y. Frère,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
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  • L. Jierry,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
    2. Université de Strasbourg, Ecole Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France
    3. International Center for Frontier Research in Chemistry, 8 allée Gaspard Monge, 67083 Strasbourg, France
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  • P. Schaaf,

    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
    2. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
    3. Université de Strasbourg, Ecole Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France
    4. International Center for Frontier Research in Chemistry, 8 allée Gaspard Monge, 67083 Strasbourg, France
    5. Institut Universitaire de France, 103 boulevard Saint-Michel, 75005 Paris, France
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  • H. Kerdjoudj,

    1. EA 4691, Université de Reims Champagne Ardenne, SFR-CapSanté, 1 avenue du Maréchal Juin, 51100 Reims, France
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  • M.-H. Metz-Boutigue,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM UMR 1121, 11 rue Humann, 67085 Strasbourg Cedex, France, Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, 67000 Strasbourg, France
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  • F. Boulmedais

    Corresponding author
    1. Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France
    2. International Center for Frontier Research in Chemistry, 8 allée Gaspard Monge, 67083 Strasbourg, France
    • Centre National de la Recherche Scientifique, Institut Charles Sadron, UPR 22, 23 rue du Loess,67034 Strasbourg Cedex 2, France.
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Abstract

Prevention of pathogen colonization of medical implants is a major medical and financial issue since infection by microorganisms constitutes one of the most serious complications after surgery or critical care. Immobilization of antimicrobial molecules on biomaterials surfaces is an efficient approach to prevent biofilm formation. Herein, the first self-defensive coating against both bacteria and yeasts is reported, where the release of the antimicrobial peptide is triggered by enzymatic degradation of the film due to the pathogens themselves. Biocompatible and biodegradable polysaccharide multilayer films based on functionalized hyaluronic acid by cateslytin (CTL), an endogenous host-defensive antimicrobial peptide, and chitosan (HA-CTL-C/CHI) are deposited on a planar surface with the aim of designing both antibacterial and antifungal coating. After 24 h of incubation, HA-CTL-C/CHI films fully inhibit the development of Gram-positive Staphylococcus aureus bacteria and Candida albicans yeasts, which are common and virulent pathogens agents encountered in care-associated diseases. Hyaluronidase, secreted by the pathogens, leads to the film degradation and the antimicrobial action of the peptide. Furthermore, the limited fibroblasts adhesion, without cytotoxicity, on HA-CTL-C/CHI films highlights a medically relevant application to prevent infections on catheters or tracheal tubes where fibrous tissue encapsulation is undesirable.

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