Formation of Functionalized Nanowires by Control of Self-Assembly Using Multiple Modified Amyloid Peptides

Authors

  • Hiroki Sakai,

    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Ken Watanabe,

    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Yuya Asanomi,

    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Yumiko Kobayashi,

    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Yoshiro Chuman,

    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Lihong Shi,

    1. Physical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
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  • Takuya Masuda,

    1. Physical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
    2. National Institute for Materials Science (NIMS), Tsukuba, 305-0044, Japan
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  • Thomas Wyttenbach,

    1. Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106 - 9510, USA
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  • Michael T. Bowers,

    1. Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106 - 9510, USA
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  • Kohei Uosaki,

    1. Physical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
    2. National Institute for Materials Science (NIMS), Tsukuba, 305-0044, Japan
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  • Kazuyasu Sakaguchi

    Corresponding author
    1. Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan
    • Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University, N10, W8, Kita-ku, Sapporo, 060-0810, Japan.

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Abstract

Amyloid peptides have great potential as building blocks in the creation of functional nanowires due to their natural ability to self-assemble into nanofibrillar structures and because they can be easily modified with various functional groups. However, significant modifications of an amyloid peptide generally alter its self-assembly property, making it difficult to construct functionalized fibrils with a desired structure and function. In this study, a very effective method to overcome this problem is demonstrated by using our structure-controllable amyloid peptides (SCAPs) terminated with a three-amino-acid-residue cap. The method consists on mixing two or more structurally related amyloid peptides with a fraction of modified SCAPs which co-assemble into a fibril. This SCAP-mixing method provides remarkable control over the self-assembly process both on the small oligomers level and the macroscopic fibrils level. Furthermore, it is shown that the modified peptides imbedded in the resulting fibril can subsequently be functionalized to generate nanowires with the desired properties, highlighting the importance of this SCAP method for nanotechnology applications.

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