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Functionalized Microparticles Producing Scaffolds in Combination with Cells

Authors

  • C. A. Custódio,

    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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  • V. E. Santo,

    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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  • M. B. Oliveira,

    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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  • M. E. Gomes,

    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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  • R. L. Reis,

    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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  • J. F. Mano

    Corresponding author
    1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Caldas das Taipas – Guimarães, Portugal
    2. ICVS/3B's, PT Government Associated Laboratory, Braga/Guimarães, Portugal
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Abstract

The development of biologically instructive biomaterials with application for tissue regeneration has become the focus of intense research over the last years. This work reports a novel approach for the production of three-dimensional constructs for tissue engineering applications based on the assembly of chitosan microparticles exhibiting specific biological response with cells. Chitosan microparticles with a size range between 20 and 70 μm are functionalized with platelet derived growth factor (PDFG-BB). The functionalization is achieved by previous immobilization of an anti-PDGF-BB antibody, using a water-soluble carbodiimide. When incubated with a cocktail of growth factors-platelet lysates, the previously functionalized particles are able to target PDGF-BB from the protein mixture. In vitro studies are carried out focusing on the ability of these systems to promote the assembly into a stable 3D construct triggered by the presence of human adipose stem cells, which act as crosslinker agents and induce the formation of a hydrogel network. The presence of immobilized growth factors gives to this system a biological functionality towards control on cell function. It is also bioresponsive, as cells drive the assembly process of the microgel. These versatile biomimetic microgels may provide a powerful tool to be used as an injectable system for non-invasive tissue engineering applications with additional control over cellular function by creating specific microenvironments for cell growth.

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