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Free-Standing Cell Sheet Assembled with Ultrathin Extracellular Matrix as an Innovative Approach for Biomimetic Tissues

Authors

  • Jun Chen,

    1. School of Basic Medical Science, Southern Medical University, Guangzhou, China
    2. Department of Mechanical and Manufacturing Engineering, University of Manitoba, Winnipeg, Canada
    3. Manitoba Institute of Child Health, Winnipeg, Canada
    4. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China
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  • Xiaozhong Qiu,

    1. School of Basic Medical Science, Southern Medical University, Guangzhou, China
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  • Leyu Wang,

    1. School of Basic Medical Science, Southern Medical University, Guangzhou, China
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  • Wen Zhong,

    1. Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
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  • Jiming Kong,

    1. School of Basic Medical Science, Southern Medical University, Guangzhou, China
    2. Department of Human Anatomy and Cell Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
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  • Malcolm M. Q. Xing

    Corresponding author
    1. School of Basic Medical Science, Southern Medical University, Guangzhou, China
    2. Department of Mechanical and Manufacturing Engineering, University of Manitoba, Winnipeg, Canada
    3. Manitoba Institute of Child Health, Winnipeg, Canada
    4. Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada
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Abstract

Current artificial tissue-substitutes have limited clinical applications due to unmatched complex combination of cells and extracellular matrix (ECM) as seen in native tissues. From a developmental perspective, the construction of effective biomimetic tissues is from the bottom (one-dimensional nanoparticles or two-dimensional membranes) up (three-dimensional scaffolds or more complex composite). In a hierarchical architecture, each sub-structure can be assembled in a flexible way with specific regulators and cells, which overcomes the deficiency of one-for-all scaffold. Here, a cell-compatible cell-lined layered nano-membrane is developed. Bioactive molecules are mounted on a nano-membrane and later released to its lined cell sheet. The cell-lined membrane is in a free-standing form to regulate cellular functions. The major advantage of this methodology is to provide a versatile approach to construct biomimetic tissues for clinical applications.

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