Electroaddressing of Cell Populations by Co-Deposition with Calcium Alginate Hydrogels (pages 2074–2080)
Xiao-Wen Shi, Chen-Yu Tsao, Xiaohua Yang, Yi Liu, Peter Dykstra, Gary W. Rubloff, Reza Ghodssi, William E. Bentley and Gregory F. Payne
Article first published online: 14 MAY 2009 | DOI: 10.1002/adfm.200900026
Calcium alginate hydrogels can be electrodeposited at electrode addresses without the need for reagents. Mechanistically, anodic signals trigger a localized solubilization of CaCO3 and the release of Ca2+ induces the spatially controlled formation of calcium alginate hydrogels. Alginate electrodeposition is sufficiently mild that bacterial cell populations co-deposited within the hydrogel are viable, grow, and respond to externally added inducers and signaling molecules.